PMSF-RO
Roche
PMSF
Phenylmethylsulfonyl fluoride
Synonym(s):
Phenylmethanesulfonyl fluoride, phenylmethylsulfonyl fluoride, α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride
About This Item
Recommended Products
Quality Level
Assay
98% (TLC)
form
solid
mol wt
174.2
packaging
pkg of 10 g (10837091001)
pkg of 25 g (11359061001)
manufacturer/tradename
Roche
concentration
17-170 μg/mL
technique(s)
western blot: suitable
pKa
7.0
mp
92-94 °C
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1 of 4
This Item | P7626 | 93482 | 52332 |
---|---|---|---|
100 | - | 200 | 200 |
solid | powder | liquid | crystalline solid |
ambient | - | - | ambient |
20-25°C | - | 2-8°C | 10-30°C |
>10 mg/mL | dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.), H2O: unstable | - | ethanol: soluble, isopropanol: soluble, methanol: soluble |
General description
Specificity
Application
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.[1][2][3]
Biochem/physiol Actions
Quality
Preparation Note
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.
Reconstitution
Other Notes
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