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PMSF-RO

Roche

PMSF

Phenylmethylsulfonyl fluoride

Synonym(s):

Phenylmethanesulfonyl fluoride, phenylmethylsulfonyl fluoride, α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride

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About This Item

Empirical Formula (Hill Notation):
C7H7FO2S
CAS Number:
Molecular Weight:
174.19
Beilstein:
2088311
MDL number:
UNSPSC Code:
12352202
PubChem Substance ID:

Pricing and availability is not currently available.

Quality Level

Assay

98% (TLC)

form

solid

mol wt

174.2

packaging

pkg of 10 g (10837091001)
pkg of 25 g (11359061001)

manufacturer/tradename

Roche

concentration

17-170 μg/mL

technique(s)

western blot: suitable

pKa 

7.0

mp

92-94 °C

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This Item
P76269348252332
PMSF Phenylmethylsulfonyl fluoride

PMSF-RO

PMSF

Quality Level

100

Quality Level

-

Quality Level

200

Quality Level

200

form

solid

form

powder

form

liquid

form

crystalline solid

shipped in

ambient

shipped in

-

shipped in

-

shipped in

ambient

storage temp.

20-25°C

storage temp.

-

storage temp.

2-8°C

storage temp.

10-30°C

solubility

>10 mg/mL

solubility

dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.), H2O: unstable

solubility

-

solubility

ethanol: soluble, isopropanol: soluble, methanol: soluble

General description

Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.

Specificity

Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.

Application

PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.[1][2][3]

Biochem/physiol Actions

PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.[4]

Quality

Purity: Chromatographically homogeneous

Preparation Note

Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.

Reconstitution

Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C.

Other Notes

Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
For life science research only. Not for use in diagnostic procedures.

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    D R Compton et al.
    The Journal of pharmacology and experimental therapeutics, 283(3), 1138-1143 (1998-02-12)
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