MABC002F
Mouse IgG1 Negative Control, clone Ci4, FITC conjugate
Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
clone
Ci4, monoclonal
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Specificity
This antibody reacts with chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.
Immunogen
Chicken immunoglobulin
Application
Fluorescein conjugated mouse IgG1 is a monoclonal antibody intended for use as a negative control in immunofluorescence and flow cytometry. Its use enables an estimation of non-specific binding of fluorescein conjugated mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.
SUGGESTED USAGE
Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.
SUGGESTED USAGE
Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.
Research Category
Secondary & Control Antibodies
Epitope Tags & General Use
Secondary & Control Antibodies
Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies
Isotype Control Antibodies
Physical form
Purified immunoglobulin. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Storage and Stability
Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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