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MAB362

Sigma-Aldrich

Anti-MAP1 Antibody, clone HM-1

ascites fluid, clone HM-1, Chemicon®

Synonym(s):

Microtubule-Associated Protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HM-1, monoclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAP1A(4130)

Specificity

Reacts specifically with MAP1 (also known as MAP 1a) on immunoblots of whole brain homogenate proteins. Shows selective staining of neurons and stronger staining of axons and dendrites in immunohistochemistry staining of brain tissue. Stains both the cell bodies and dendritic trees. No cross-reactivity observed to other MAPs or tubulin.

Immunogen

Rat brain microtubule-associated protein.

Application

Detect MAP1 using this Anti-MAP1 Antibody, clone HM-1 validated for use in WB, IC, IH.
Immunoblotting at 1:500 using fresh rat brain extract or enriched microtubule protein preparation.

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Physical form

Ascites fluid. Liquid containing 15mM sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alejandra Bosco et al.
The Journal of comparative neurology, 519(4), 599-620 (2011-01-20)
Changes in microglial cell activation and distribution are associated with neuronal decline in the central nervous system (CNS), particularly under pathological conditions. Activated microglia converge on the initial site of axonal degeneration in human glaucoma, yet their part in its
Lihai Zhang et al.
Toxicological sciences : an official journal of the Society of Toxicology, 117(1), 180-189 (2010-06-18)
2,5-Hexanedione (HD) intoxication is associated with axon atrophy that might be responsible for the characteristic gait abnormalities, hindlimb skeletal muscle weakness and other neurological deficits that accompany neurotoxicity. Although previous mechanistic research focused on neurofilament triplet proteins (NFL, NFM, NFH)
Deregulated Transcription and Proteostasis in Adult mapt Knockout Mouse.
Andr??s-Benito, et al.
International Journal of Molecular Sciences, 24 (2023)
Kristin Kathleen Baxter et al.
ASN neuro, 1(4) (2009-09-12)
Mitochondria play a central role during neurogenesis by providing energy in the form of ATP for cytoskeletal remodelling, outgrowth of neuronal processes, growth cone activity and synaptic activity. However, the fundamental question of how differentiating neurons control mitochondrial biogenesis remains
Erin M Masucci et al.
Molecular biology of the cell, mbcE21100480-mbcE21100480 (2021-10-28)
Microtubules establish the directionality of intracellular transport by kinesins and dynein through polarized assembly, but it remains unclear how directed transport occurs along microtubules organized with mixed polarity. We investigated the ability of the plus-end directed kinesin-4 motor KIF21B to

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