MAB1922
Anti-Laminin α2 Antibody, clone 5H2
ascites fluid, clone 5H2, Chemicon®
Synonym(s):
Laminin M chain, Merosin heavy chain, laminin M, laminin alpha 2 subunit, laminin, alpha 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
5H2, monoclonal
species reactivity
human, rabbit, monkey
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
cell culture | mammalian: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... LAMA2(3908)
rhesus monkey ... Lama2(715394)
General description
Elements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14 - 19 with multiple developmental abnormalities. The kidney phenotypes include avascular glomeruli, impaired branching morphogenesis, and renal agenesis.
Specificity
Cross reacts with monkey and rabbit merosin.
Reacts with the 80 kDa fragment of the M-chain of human merosin.
Immunogen
Purified human merosin
Application
Detect Laminin α2 using this Anti-Laminin α2 Antibody, clone 5H2 validated for use in ELISA, CULT, IF, IH, IP & WB.
ELISA:
50% maximal binding to human merosin at 1:50,000 dilution from a previous lot.
Immunohistochemistry:
1:5,000 dilution from a previous lot was used for staining of 8 µm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence:
A previous lot of this antibody was used in immunofluorescent.
Affinity chromatography:
A previous lot of this antibody was used in IAP.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
50% maximal binding to human merosin at 1:50,000 dilution from a previous lot.
Immunohistochemistry:
1:5,000 dilution from a previous lot was used for staining of 8 µm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence:
A previous lot of this antibody was used in immunofluorescent.
Affinity chromatography:
A previous lot of this antibody was used in IAP.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Quality
Routinely evaluated by Western Blot on Human Placenta lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate.
Target description
80 kDa
Physical form
Unpurified
Unpurified ascites in buffer containing no preservatives.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
Analysis Note
Control
Vascular breast carcinomas, Cultured embryonic retinal neurons and RGCs
Vascular breast carcinomas, Cultured embryonic retinal neurons and RGCs
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ying Gao et al.
Endocrinology, 158(4), 963-978 (2017-03-23)
A local axis connects the apical ectoplasmic specialization (ES) at the Sertoli-spermatid interface, the basal ES at the blood-testis barrier (BTB), and the basement membrane across the seminiferous epithelium functionally in rat testes. As such, cellular events that take place
Holly Colognato et al.
Development (Cambridge, England), 134(9), 1723-1736 (2007-03-31)
Developmental abnormalities of myelination are observed in the brains of laminin-deficient humans and mice. The mechanisms by which these defects occur remain unknown. It has been proposed that, given their central role in mediating extracellular matrix (ECM) interactions, integrin receptors
Isabelle Nelson et al.
Journal of neuromuscular diseases, 2(3), 229-240 (2015-09-02)
Laminin α2 deficient congenital muscular dystrophy, caused by mutations in the LAMA2 gene, is characterized by early muscle weakness associated with abnormal white matter signal on cerebral MRI. To report on 4 patients with LAMA2 gene mutations whose original clinical
Zia A Khan et al.
The Journal of clinical investigation, 118(7), 2592-2599 (2008-06-07)
Infantile hemangioma is a benign endothelial tumor composed of disorganized blood vessels. It exhibits a unique life cycle of rapid postnatal growth followed by slow regression to a fibrofatty residuum. Here, we have reported the isolation of multipotential stem cells
Heike Kölbel et al.
Frontiers in neurology, 10, 470-470 (2019-05-28)
Laminin-211 deficiency leads to the most common form of congenital muscular dystrophy in childhood, MDC1A. The clinical picture is characterized by severe muscle weakness, brain abnormalities and delayed motor milestones defining MDC1A as one of the most severe forms of
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