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EZRGRA

Millipore

Rat/Mouse Ghrelin ELISA Kit

measures and quantifies active Ghrelin in 20 μL serum or plasma

Synonym(s):

Appetite-regulating hormone

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

Pricing and availability is not currently available.

product name

Rat/Mouse Ghrelin (active) ELISA, This Rat/Mouse Ghrelin (active) ELISA is used to measure & quantify Ghrelin levels in Metabolism & Endocrine research.

Quality Level

species reactivity

rat, mouse

packaging

kit of 1 × 96 wells

parameter

20 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma (K2 EDTA)

application(s)

research use

assay range

inter-assay cv: 1-10%
intra-assay cv: 0.3-7%
(Serum)

inter-assay cv: 1-5%
intra-assay cv: 1-6%
(Plasma)

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This Item
B7653SAB4200680A0185
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

agarose conjugate

conjugate

unconjugated

conjugate

-

conjugate

peroxidase conjugate

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

capture ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: 1:4,000

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.15-0.3 μg/mL, western blot: suitable

technique(s)

dot blot: suitable, flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, indirect ELISA: 1:30,000, western blot (chemiluminescent): 1:80,000

General description

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.

Millipore is pleased to offer a new Rat/Mouse Ghrelin (Active) ELISA kit for the quantification of active ghrelin in rat and mouse serum or plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.

Specificity

Rat/Mouse Ghrelin (Active) - 100%
Rat/Mouse "Des-Octanoyl" Ghrelin - 0%
Canine Ghrelin (Active) - 111%
Porcine Ghrelin (Active) - 98%

Application

Used to detect/quantify: Ghrelin

Other Notes

Please contact Technical Service for linearity of dilution.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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Sang-Mi Woo et al.
Molecular medicine reports, 17(2), 2665-2672 (2017-12-06)
Chemotherapy frequently causes anorexia in cancer patients, which has been associated with poor disease prognosis. Several therapeutic strategies for the treatment of chemotherapy‑induced anorexia are available; however, their adverse effects limit their clinical use. Herbal medicines have a long history
Ghrelin ameliorates hypoxia-induced pulmonary hypertension via phospho-GSK3{beta}{beta}-catenin signaling in neonatal rats.
Xu YP, Zhu JJ, Cheng F, Jiang KW, Gu WZ, Shen Z, Wu YD, Liang L, Du LZ
Journal of Molecular Endocrinology null
Diet-induced obesity causes ghrelin resistance in arcuate NPY/AgRP neurons.
Briggs DI, Enriori PJ, Lemus MB, Cowley MA, Andrews ZB
Endocrinology null

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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