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CC065

Sigma-Aldrich

Human Tenascin

from human glioma cell line (U251), liquid, 0.1 mg/mL, suitable for cell culture

Synonym(s):

TN-C, Tenascin, Hexabrachion, Cytotactin, Neuronectin

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

Human Tenascin-C Purified Protein,

biological source

human

Quality Level

100
300

Assay

>97% (SDS-PAGE)

form

liquid

mol wt

280—300 kDa

manufacturer/tradename

Chemicon®

concentration

0.1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)

solubility

water: soluble

NCBI accession no.

NM_002160.2

UniProt accession no.

P24821

Binding Specificity

Peptide Source: Fibrinogen

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TNC(3371)

General description

Product Source: Human glioma cell line (U251).
Purified Human Tenascin-C Protien. Tenascin is known to: 1) play an active role in development of central nervous system and mesenchymal-derived organs; 2) exist in vasculature of tumors in adults; and 3) function in cell adhesion. By SDS-PAGE analysis, the protein migrates around 280-300kD.

Application

Cell adhesion assays.

Optimal working dilutions must be determined by end user.

Linkage

Replaces: CC066

Physical form

Purified protein supplied as a liquid in PBS containing no preservatives.

Storage and Stability

Maintain at -70ºC for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Longui Ning et al.
BioMed research international, 2019, 1475705-1475705 (2019-12-31)
Tenascin-C (TNC) is an extracellular matrix glycoprotein expressed in response to inflammation and tissue damage. The role of TNC in patients with inflammatory bowel disease (IBD) is not well understood. In this study, we analyzed the expression of TNC in
Azhar Maqbool et al.
World journal of cardiology, 8(5), 340-350 (2016-05-28)
To investigate the effect of Tenascin C (TNC) on the expression of pro-inflammatory cytokines and matrix metalloproteinases in human cardiac myofibroblasts (CMF). CMF were isolated and cultured from patients undergoing coronary artery bypass grafting. Cultured cells were treated with either
So-Young Yeo et al.
Nature communications, 9(1), 3016-3016 (2018-08-03)
Although fibroblasts are dormant in normal tissue, they exhibit explosive activation during wound healing and perpetual activation in pathologic fibrosis and cancer stroma. The key regulatory network controlling these fibroblast dynamics is still unknown. Here, we report that Twist1, a
Susobhan Sarkar et al.
Cancer research, 77(12), 3231-3243 (2017-04-19)
Oncogenic signaling by NOTCH is elevated in brain tumor-initiating cells (BTIC) in malignant glioma, but the mechanism of its activation is unknown. Here we provide evidence that tenascin-C (TNC), an extracellular matrix protein prominent in malignant glioma, increases NOTCH activity
Janeen H Trembley et al.
Cancer letters, 315(1), 48-58 (2011-11-05)
CK2, a pleiotropic Ser/Thr kinase, is an important target for cancer therapy. We tested our novel tenfibgen-based nanocapsule for delivery of the inhibitor 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole (DMAT) and an siRNA directed against both CK2α and α' catalytic subunits to prostate cancer cells.
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