AB10910
Anti-Osteopontin Antibody
serum, from rabbit
Synonym(s):
Bone sialoprotein 1, SPP1/CALPHA1 fusion, Urinary stone protein, bone sialoprotein I, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, e
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SPP1(6696)
General description
Osteopontin is a single-chain polypeptide. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling. Expression of osteopontin in a variety of tissues indicates a multiplicity of functions that involve one or more of these conserved motifs. While the lack of a clear phenotype in osteopontin "knockout" mice has not established a definitive role for osteopontin in any tissue, recent studies have provided some novel and intriguing insights into the versatility of this enigmatic protein in diverse biological events, including developmental processes, wound healing, immunological responses, tumorigenesis, bone resorption, and calcification. The ability of osteopontin to stimulate cell activity through multiple receptors linked to several interactive signaling pathways can account for much of the functional diversity.
Specificity
The antibody reacts with the 60 kDa osteopontin protein.
Immunogen
Epitope: aa 75-90
Synthetic peptide corresponding to aa 75-90 of osteopontin protein.
Application
Detect Osteopontin using this Anti-Osteopontin Antibody validated for use in WB, IH.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Quality
Routinely evaluated by Western Blot on mouse kidney lysate.
Western Blot Analysis:
1:500 - 1:5,000 dilution of this antibody detected osteopontin in 10 μg of mouse kidney lysate.
Western Blot Analysis:
1:500 - 1:5,000 dilution of this antibody detected osteopontin in 10 μg of mouse kidney lysate.
Target description
60 kDa
Physical form
Serum containing 0.05% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Mouse kidney, COS-1 and human urine lysate
Mouse kidney, COS-1 and human urine lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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