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Casein Kinase II, Human, Recombinant, E. coli

Casein Kinase II, Human, Recombinant, E. coli Recombinant, is expressed in E. coli. A cyclic nucleotide-independent Ser/Thr protein kinase composed of catalytic α-subunits and regulatory β-subunits.

Synonym(s):

rhCKII

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in E. coli

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Research area: CELL SIGNALING

Note: 1 KU = 1000 units.

Recombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits and regulatory β-subunits in an α2β2- tetramer. This material is one of several forms of native CK-II. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies. The following substrates have been characterized by CK-II both in vitro and in vivo: nucleolin, RNA polymerase, p53, and SV 40 large T antigen.

Application

Casein kinase II (CK2) has been used:
  • has been used as a marker for CK2 subunits, to analyze CK2 expression in human retinal extract.
  • to investigate the phosphorylation of the C-terminal domain of uroplakin IIIa and assess its potential regulation by CK2.
  • to investigate the phosphorylation events and their impact on spectrin tetramer formation and neuronal cell function.

Biochem/physiol Actions

Casein kinase II (CK2) is an active serine/threonine protein kinase that plays a pivotal role in cell growth and division. It is responsible for phosphorylating various nuclear proteins involved in DNA metabolism, transcription factors, and oncogenes, thus influencing their activity. It also regulates osteogenesis, adipogenesis, chondrogenesis, neuron differentiation, and immune response pathways. CK2 plays a crucial role in activating the Janus Kinase/signal transducer and activator of transcription (JAK/STAT) pathway, which contributes to cell survival, differentiation, and proliferation. Furthermore, CK2 is involved in activating the nuclear factor kappa B (NF-κB) pathway. It is highly expressed in different hematological malignancies, including acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), and chronic myeloid leukemia (CML).

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.

Physical form

In 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON X-100 Detergent, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Yamaguchi, Y., et al. 1998. Nucleic Acids Res.26, 3854.
Chester, N., and Marshak, D.R. 1993. Anal. Biochem.209, 284.
Russo, G.L., et al. 1992. J. Biol. Chem.267, 20317.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Converting GTP hydrolysis into motion: versatile translational elongation factor G
Rodnina MV, et al.
Biological Chemistry, 401(1), 131-142 (2019)
Bacteria-induced uroplakin signaling mediates bladder response to infection
Thumbikat P, et al.
PLoS Pathogens, 5(5), e1000415-e1000415 (2009)
Phosphorylation of a threonine unique to the short C-terminal isoform of betaII-spectrin links regulation of alpha-beta spectrin interaction to neuritogenesis
Bignone PA, et al.
The Journal of Biological Chemistry, 282(2), 888-896 (2007)
Expression of protein kinase CK2 in astroglial cells of normal and neovascularized retina
Kramerov A, et al.
The American Journal of Pathology, 168(5), 1722-1736 (2006)
The Role of Protein Kinase CK2 in Development and Disease Progression: A Critical Review
Halloran D, et al.
Journal of developmental biology, 10(3), 31-31 (2022)

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