While most HPLC columns feature 10.32 threaded end fittings, some may have different types and sizes of fittings. Given the wide variety of HPLC systems, it is not possible to guarantee with complete certainty that a specific HPLC system utilizes 10.32 fittings. Furthermore, even within a single type of fitting, such as 10.32, there are short, standard, and extended versions of the nut and ferrule. Hence, it is advisable to consult the instrument manual to determine the correct fitting type and size.
1.50247
Purospher® STAR NH2 (5 µm) HPLC Columns
L × I.D. 150 mm x 4.6 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use
Synonym(s):
Purospher® STAR NH2 (5 µm) LiChroCART™ 150-4.6
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About This Item
UNSPSC Code:
41115709
NACRES:
SB.52
Pricing and availability is not currently available.
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Product Name
Purospher® STAR NH2 (5 µm), L × I.D. 150 mm x 4.6 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use
material
stainless steel column
Quality Level
Agency
suitable for USP L8
product line
Purospher®
feature
endcapped: no
packaging
1 ea of
extent of labeling
3.5% Carbon loading
parameter
250 bar pressure
60 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
15 cm × 4.6 mm
surface area
330 m2/g
matrix
fully porous particle
matrix active group
NH2 bonding phase
particle size
5 μm
pore size
120 Å pore size
operating pH range
2-7.5
separation technique
hydrophilic interaction (HILIC)
normal phase
reversed phase
storage temp.
no temp limit
Related Categories
General description
Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phaseconsisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART™ 150-4.6 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART™ 4-4 part number 1.50267.0001. LiChroCART™ cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 150 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide
Application
Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.
Features and Benefits
- Ultra-pure silica (99.999%)
- Low silanol activity
- Reproducible results from run-to-run and batch-to-batch
Analysis Note
Pressure: ≤ 45 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0
Legal Information
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Is the product Purospher® STAR NH2 (5 µm) LiChroCART® 150-4.6 column (catalog number 1.50247.0001 or 48219-474) compatible with the Agilent 1260 HPLC system?
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