1.46000

HEIMPLATE Violet Red Bile Dextrose Agar Settle plate

Heimplate, APHA, ISO 21528, USP, JP, plate diam. × filling volume 90 mm × 30 mL/ft

• Synonym(s): VRBD, Violet Red Bile Glucose agar, Crystal Violet Neutral Red Bile Glucose agar
• UNSPSC Code: 41106217
• NACRES: NA.74

Properties

• Agency: APHA; EP; ISO 21528; JP; USP
• Quality Level: 300
• sterility: sterile
• form: plate (ready-to-use)
• feature: ready-to-use; ICR Settle plate
• manufacturer/tradename: Heimplate
• packaging: pkg of (20 or 120 plates per box)
• technique(s): microbiological culture: suitable
• plate diam. × filling volume: 90 mm × 30 mL/ft
• pH: 7.4 ( in H₂O)
• application(s): microbiology; pharmaceutical; surface monitoring
• storage temp.: 15-25°C
• suitability: Escherichia coli; Salmonella spp.; coliforms

Description

General description

Violet Red Bile Dextrose (VRBD) Agar settle plates are used for the detection and enumeration of Enterobacteriaceae and bile-tolerant Gram-negative bacteria. Crystal violet and bile salts inhibit the accompanying bacterial flora. Fermentation of glucose by Enterobacteriaceae leads to acid production, which is indicated by a color change to red and by zones of precipitated bile acids surrounding the colonies. Enzymatic digest of animal tissue provides carbon and nitrogen sources for the growth, and yeast extract primarily supplies the B-complex vitamins. The culture medium is not specific for Enterobacteriaceae as some other accompanying bacteria (e.g. Aeromonas) also show the same reaction.

Application

Violet Red Bile Dextrose (VRBD) Agar settle plates are used to detect and enumerate Enterobacteriaceae from food, animal feed, and other materials. They are also suitable for the enumeration of bile-tolerant Gram-negative bacteria from pharmaceuticals and other materials.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2