07-175

Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): JNK1 alpha protein kinase, JNK1 beta protein kinase, JUN N-terminal kinase, Stress-activated protein kinase JNK1, Stress-activated protein kinase JNK1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-ac
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, horse
• species reactivity (predicted by homology): canine (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), equine (based on 100% sequence homology)
• technique(s): activity assay: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_620634
• UniProt accession no.: P45983
• shipped in: wet ice
• target post-translational modification: phosphorylation (p(Thr183/pTyr185), (pThr221/pTyr223))
• Gene Information: human ... MAPK8(5599)

Description

General description

JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.

Specificity

This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .

Immunogen

Epitope: Phosphorylated at Thr183/Tyr185 and Thr221/Tyr223

KLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223.

Application

Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate.

Research Category
Signaling

Research Sub Category
MAP Kinases

Quality

Evaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.

Target description

~ 42 kDa observed. There are several isoforms between 40-50 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Untreated and Lambda phosphatase treated RAW264.7 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1