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06-984

Sigma-Aldrich

Anti-Mn-SOD Antibody

Upstate®, from rabbit

Synonym(s):

Mn superoxide dismutase, indophenoloxidase B, manganese superoxide dismutase, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, bovine, human, rat

should not react with

canine

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_000627

UniProt accession no.

P04179

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... SOD2(6648)

General description

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.

Specificity

Recognizes MnSOD, Mr 24 kDa.

Immunogen

Recombinant human MnSOD (manganous superoxide dismutase).

Application

Anti-Mn-SOD Antibody is an antibody against Mn-SOD for use in IH, IH(P) & WB.
Immunohistochemistry (Paraffin):
10 μg/mL of a previous lot detected MnSOD in paraffin embedded rat brain tissue sections.

Quality

Evaluated by western blot on RIPA lysates of MCF-7, A431 or rat L6 RIPA cells.

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected MnSOD in RIPA lysates of rat L6 RIPA cells. 1-4 μg/mL of a previous lot detected MnSOD in RIPA lysates of human MCF-7, A431 and rat L6 RIPA cells.

Target description

~24 kDa

Physical form

Format: Purified
Purified rabbit polyclonal in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Analysis Note

Control
L6 cell lysate, rat brain tissue extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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