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Key Documents

05-599

Sigma-Aldrich

Anti-E1A (Ad2/Ad5) Antibody, clone M73

clone M73, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M73, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Specificity

three adenovirus (Ad) 2 and 5 E1A gene products

Immunogen

trpE-Ad2 E1A fusion protein isolated from E. coli

Application

Detect E1A (Ad2/Ad5) with Anti-E1A (Ad2/Ad5) Antibody, clone M73 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

routinely evaluated by immunoblot on in RIPA lysates from HEK293 cells

Target description

36-50kDa

Physical form

0.05M sodium phosphate, pH 7.4, 0.1% sodium azide and 0.2% gelatin
Format: Purified
Protein A chromatography

Storage and Stability

1 year at 4°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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David F Bodenstein et al.
Stem cell research & therapy, 14(1), 202-202 (2023-08-15)
Mitochondrial dysfunction is involved in several diseases ranging from genetic mitochondrial disorders to chronic metabolic diseases. An emerging approach to potentially treat mitochondrial dysfunction is the transplantation of autologous live mitochondria to promote cell regeneration. We tested the differential filtration-based
Positive and negative effects of adenovirus type 5 helper functions on adeno-associated virus type 5 (AAV5) protein accumulation govern AAV5 virus production.
Nayak, R; Pintel, DJ
Journal of virology null
Vibhu Prasad et al.
ACS infectious diseases, 3(6), 398-405 (2017-04-25)
Human adenoviruses (HAdVs) infect respiratory, gastrointestinal, and urinary tracts and give rise to eye infections and epidemic keratoconjunctivitis (EKC). They persist in lymphoid tissue and cause morbidity and mortality in immunocompromised people. Treatments with significant postexposure efficacy are not available.
E Harlow et al.
Journal of virology, 55(3), 533-546 (1985-09-01)
Hybridomas secreting monoclonal antibodies specific for the adenovirus early region 1A (E1A) proteins were prepared from BALB/c mice immunized with a bacterial trpE-E1A fusion protein. This protein is encoded by a hybrid gene that joins a portion of the Escherichia
Eiji Toyoda et al.
Journal of experimental & clinical cancer research : CR, 27, 30-30 (2008-08-23)
To develop a novel therapeutic strategy for human pancreatic cancer using a midkine promoter-based conditionally replicating adenovirus. We examined midkine mRNA expression and midkine protein expression by seven human pancreatic cancer cell lines (AsPC-1, BxPC-3, CFPAC-1, HPAC, MIAPaCa-2, PANC-1, and

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