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219215

Sigma-Aldrich

Leucocrystal Violet

Synonym(s):

4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet

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About This Item

Linear Formula:
CH[C6H4N(CH3)2]3
CAS Number:
603-48-5
Molecular Weight:
373.53
EC Number:
210-043-9
MDL number:
MFCD00008314
UNSPSC Code:
12171500
PubChem Substance ID:
24853114
NACRES:
NA.47

form

powder

technique(s)

microbe id | staining: suitable

mp

175-177 °C (lit.)

λmax

260 nm

ε (extinction coefficient)

≥700 at 257-263 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3

InChI key

OAZWDJGLIYNYMU-UHFFFAOYSA-N

Application

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

Biochem/physiol Actions

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.

Caution

Photo-ionization can cause color variations

related product

Product No.
Description
Pricing

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H410

Precautionary Statements

P273 - P391 - P501

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000236216
MKBW1177V
MKBR8820V
MKBR8440V
S41106

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L G Rushing et al.
Journal of chromatography. B, Biomedical sciences and applications, 688(2), 325-330 (1997-01-24)
A sensitive analytical procedure for the determination of residues of leucomalachite green (LMG)-malachite green (MG) and leucogentian violet (LGV)-gentian violet (GV) in catfish or trout tissue is presented. Frozen (-20 degrees C) fish fillets were cut into small pieces and
Use of leuco crystal violet to enhance shoe prints in blood.
Bodziak WJ
Forensic Science International, 82, 45-52 (1996)
Detection Method for Histamine-Producing, Dairy-Related
Bacteria using Diamine Oxidase and Leucocrystal Violet
SUMNER S S
Journal of Food Protection, 52, 105-108 (1989)
D R Doerge et al.
Rapid communications in mass spectrometry : RCM, 10(12), 1479-1484 (1996-01-01)
Gentian violet (GV) is a triphenylmethane dye antiseptic with potential for illegal use in livestock production, especially aquaculture where the related malachite green has been widely used. This potential misuse has regulatory importance because of the observed rodent carcinogenicity of
Craig A. Coppock
Contrast: An Investigator's Basic Reference Guide to Fingerprint Identification Concepts (2007)
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