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198250

Sigma-Aldrich

Rose bengal

certified by the Biological Stain Commission, Dye content ≥80 %

Synonym(s):

4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal sodium salt

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About This Item

Empirical Formula (Hill Notation):
C20H2Cl4I4Na2O5
CAS Number:
632-69-9
Molecular Weight:
1017.64
Colour Index Number:
45440
Beilstein:
3645857
EC Number:
211-183-3
MDL number:
MFCD00005043
UNSPSC Code:
12171500
PubChem Substance ID:
24851870
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

100

form

powder

composition

Dye content, ≥80%

color

dark violet

solubility

H2O: 1 mg/mL

λmax

548 nm

ε (extinction coefficient)

≥90000 at 546-550 nm at 0.008 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]C(=O)c1c(Cl)c(Cl)c(Cl)c(Cl)c1C2=C3C=C(I)C(=O)C(I)=C3Oc4c(I)c([O-])c(I)cc24

InChI

1S/C20H4Cl4I4O5.2Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;/h1-2,29H,(H,31,32);;/q;2*+1/p-2

InChI key

UWBXIFCTIZXXLS-UHFFFAOYSA-L

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General description

Rose Bengal is the sodium salt of tetraiodotetrachlorouorescein, C20H2O5I4Cl4Na2. It is an anionic water-soluble xanthene dye.

Application

Rose bengal has several microbilogical staining applications including:
  • Conn′s technique for bacteria in soil and Kreyberg′s stain for keratin and mucus
  • counterstaining hematoxylin
  • staining cellular inclusions, together with Bismark brown



Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS1232
0000253671
SHBQ2115
SHBP3528
SHBN9281

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