This product does not have a specification that describes "mg of beads per one milliliter." The product is described as a "~ 4 mg/mL protein-to-beads ratio" meaning there is approximately 4 mg of antibody conjugated to 1 mL of beads. This is a description of the product and not a recommendation for use. The general guidance provided in the technical bulletin suggests using 20 µL of the anti-HA magnetic agarose beads, which have an estimated bead capacity of 50 µg. It is suggested to add a sufficient amount of cell lysate where 50 µg of the HA-tagged protein is expected to be present.
SAE0197
Anti-HA Magnetic Beads
Magnetic agarose, suspension
Synonym(s):
HA beads
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.56
Pricing and availability is not currently available.
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General description
Anti-HA Magnetic Beads are a conjugate of high-affinity mouse IgG1 monoclonal anti-HA antibody against HA tag (YPYDVPDYA) derived from human influenza the hemagglutinin (HA) protein. The high degree of charged amino acids in the HA-tag enhances its potential for strong antibody recognition. This product may be used for purification of HA-tagged proteins that are expressed in bacterial and mammalian cell lysates.
Application
Features and Benefits
- Antibody is conjugated to cyanogen bromide-activated magnetic agarose beads.
- Protein-to-beads ratio ~ 4 mg/mL.
- High specificity binding to HA-tagged proteins.
Other Notes
This product is for R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
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Control of histone demethylation by nuclear-localized ?-ketoglutarate dehydrogenase
Huang F, et al.
Plant Science, 381 (6654) (2023)
BMI1 induces ubiquitination and protein degradation of Nod-like receptor family CARD domain containing 5 and suppresses human leukocyte antigen class I expression to induce immune escape in non-small cell lung cancer
Zhi-Hui Lu, et al.
The Kaohsiung Journal of Medical Sciences, 38(12) (2022)
Zhi-Hui Lu et al.
The Kaohsiung journal of medical sciences, 38(12), 1190-1202 (2022-10-05)
The Nod-like receptor (NLR) family CARD domain containing 5 (NLRC5) has been reported as an activator of human leukocyte antigen (HLA) class I that is responsible for immune activity in cancer treatment. This work focuses on the role of BMI1
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The beads are supplied as the suspension and the loading is described as 4mg of antibody for 1mg of beads. However the concentration of suspension is not specified. How many mg of beads are there in the 1ml of supplied suspension?
1 answer-
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What is the average size of the beads?
1 answer-
This product consists of a non-crosslinked magnetic agarose resin with a bead size of 20-75 µm.
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Is the antibody covalently conjugated to the beads and will the HA-antibody comes off during elution or boiling?
1 answer-
In SAE0197, the antibody is covalently conjugated to the beads, and the HA-antibody remains bonded to the beads during elution or boiling.
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