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SAE0197

Sigma-Aldrich

Anti-HA Magnetic Beads

Magnetic agarose, suspension

Synonym(s):

HA beads

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
Pricing and availability is not currently available.

conjugate

magnetic beads

Quality Level

200

form

suspension

shipped in

wet ice

storage temp.

2-8°C

General description

Anti-HA Magnetic Beads are a conjugate of high-affinity mouse IgG1 monoclonal anti-HA antibody against HA tag (YPYDVPDYA) derived from human influenza the hemagglutinin (HA) protein. The high degree of charged amino acids in the HA-tag enhances its potential for strong antibody recognition. This product may be used for purification of HA-tagged proteins that are expressed in bacterial and mammalian cell lysates.

Application

These anti-HA magnetic agarose beads may be used in various immunological techniques, such as immunoprecipitation[1], immunoaffinity purification, ubiquitination assay.[2]

Features and Benefits

  • Antibody is conjugated to cyanogen bromide-activated magnetic agarose beads.
  • Protein-to-beads ratio ~ 4 mg/mL.
  • High specificity binding to HA-tagged proteins.

Other Notes

This product is for R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000382607
0000329598
0000382607
0000329598
0000251303

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Control of histone demethylation by nuclear-localized ?-ketoglutarate dehydrogenase
Huang F, et al.
Plant Science, 381 (6654) (2023)
Zhi-Hui Lu et al.
The Kaohsiung journal of medical sciences, 38(12), 1190-1202 (2022-10-05)
The Nod-like receptor (NLR) family CARD domain containing 5 (NLRC5) has been reported as an activator of human leukocyte antigen (HLA) class I that is responsible for immune activity in cancer treatment. This work focuses on the role of BMI1
BMI1 induces ubiquitination and protein degradation of Nod-like receptor family CARD domain containing 5 and suppresses human leukocyte antigen class I expression to induce immune escape in non-small cell lung cancer
Zhi-Hui Lu, et al.
The Kaohsiung Journal of Medical Sciences, 38(12) (2022)

Questions

1–2 of 2 Questions  

  1. What is the average size of the beads?

    1 answer

    1. This product consists of a non-crosslinked magnetic agarose resin with a bead size of 20-75 µm.

      Helpful?

  2. Is the antibody covalently conjugated to the beads and will the HA-antibody comes off during elution or boiling?

    1 answer

    1. In SAE0197, the antibody is covalently conjugated to the beads, and the HA-antibody remains bonded to the beads during elution or boiling.

      Helpful?

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