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Key Documents

SAB5700611

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody

rabbit polyclonal

Synonym(s):

ALXDRD, GFAP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

Product Name

Anti-GFAP antibody produced in rabbit,

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

49
 50

species reactivity

human, mouse, rat

concentration

2.32 mg/ml

technique(s)

immunofluorescence: 1:50-1:200
western blot: 1:500-1:2000

UniProt accession no.

P14136

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)

General description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).

Physical form

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Storage and Stability

Store at -20℃. Avoid freeze / thaw cycles.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
3560492113
3560492111

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Questions

  1. こんにちは、マウスのアストロサイトにおけるGFAP含有量を測定したいと考えています。免疫蛍光法を使用する予定で、現在Anti-GFAP （SAB5700611）とAnti-Rabbit IgG (H+L)（SAB4600045）の2つの抗体を選んでいます。これらの抗体は理論的には相互に結合できますか？ また、これら2つの抗体の推奨濃度についても教えていただけると嬉しいです。 よろしくお願いいたします。

    1 answer

    1. Anti-GFAP (SAB5700611) is a primary antibody targeting the Glial fibrillary acidic protein found in astrocytes. When used in conjunction with Anti-Rabbit IgG (H+L) (SAB4600045) in an experiment, e.g., immunofluorescence or western blot, the goat-produced Anti-Rabbit IgG (H+L) secondary antibody (SAB4600045) would bind to the rabbit-produced Anti-GFAP primary antibody. This is because the secondary antibody is specific for rabbit immunoglobulins, and the primary antibody is produced in rabbits, thus containing rabbit IgG.
      Regarding the working concentrations, see the dilutions factors for each item below. Please note that this information is also listed in the Properties section of the product page.
      SAB5700611
      immunofluorescence: 1:50-1:200
      western blot: 1:500-1:2000

      SAB4600045
      flow cytometry: 1-10 μg/mL
      immunocytochemistry: suitable
      immunohistochemistry: suitable
      indirect immunofluorescence: 1-10 μg/mL

      Helpful?

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