SAB4600050

Monoclonal Anti-Fluorescein (FITC) IgG, CF^™ 488A antibody produced in mouse

~2 mg/mL, clone 1F8.-1E4, purified immunoglobulin

• Synonym(s): Anti Fluorescein Antibody, Anti Fluorescein Antibody - Monoclonal Anti-Fluorescein (FITC) IgG, CF^™488A antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: CF^™ 488A conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1F8.-1E4, monoclonal
• form: buffered aqueous solution
• concentration: ~2 mg/mL
• technique(s): indirect immunofluorescence: 1-10 μg/mL
• isotype: IgG1κ
• fluorescence: λ_ex 490 nm; λ_em 515 nm
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and consists of a gamma (g) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. The monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with a molecular weight of 50kDa and 25kDa respectively, the primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named g1,g2, g3, and g4, respectively. IgG is produced and released from CD5+B cells, the predominant lymphocytes in the neonatal cell repertoire.

Specificity

Binds fluorescein

Immunogen

fluorescein

Application

Monoclonal Anti-Fluorescein (FITC) IgG, CF^™ 488A antibody produced in mouse has been used in Off-chip miRNA targeting and On-chip miRNA cleavage.

Biochem/physiol Actions

Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.
CF^™ dyes are a series of highly water-soluble fluorescent dyes, with wavelength extended over the visible and near-infrared (IR) spectrum for labeling biomolecules, mainly proteins and nucleic acids. The primary structure of the CF^™ dyes is either cyanine dyes or rhodamine dyes. CF^™ 633 produces the brightest antibody conjugates among spectrally similar dyes when excited by the 633nm He-Ne laser or the 635nm red diode laser. The most important advantage of CF^™ 633 is its excellent photostability. The molecular weight of CF^™ 633 is ~820 and its absorption/emission maxima are at 630/650nm.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

Preparation Note

Protect from light.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2