The isotype of this product, Anti-IDH1 (R132H) antibody, Mouse monoclonal, is IgG. It has not been further determined if it is IgG1 or IgG2, or if it has a lambda or kappa chain.
SAB4200548
Anti-IDH1 (R132H) antibody, Mouse monoclonal
clone HMab-1, purified from hybridoma cell culture
Synonym(s):
Idh1 R132H Antibody, Idh1 R132H Antibody - Anti-IDH1 (R132H) antibody, Mouse monoclonal, Anti-IDCD, Anti-IDH, Anti-IDP, Anti-IDPC, Anti-Isocitrate dehydrogenase 1 (NADP+), soluble, Anti-NADP(+)-specific ICDH, Anti-NADP-dependent isocitrate dehydrogenase, cytosolic, Anti-NADP-dependent isocitrate dehydrogenase, peroxisomal, Anti-Oxalosuccinate decarboxylase, Anti-PICD
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HMab-1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~43 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
western blot: 4.0-8.0 μg/mL using extract of HEK-293T cells overexpressing IDH1R132H.
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IDH1(3417)
General description
Anti-IDH1 (R132H) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma HMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to mutation R132H of human IDH1. A member of isocitrate dehydrogenase (IDH) family, IDH1, is the human cytoplasmic NADP-specific enzyme. Its subcellular localization was shown to be in both peroxisomes and the cytoplasm.
Immunogen
peptide corresponding to mutation R132H of human IDH1.
Application
Anti-IDH1 (R132H) antibody has been used:
- in immunoblotting
- in immunohistochemistry
- in western blotting
- in cell cycle analysis and apoptosis assays
- in chromatin immunoprecipitation (ChIP) assay
- in in vitro migration assay
Biochem/physiol Actions
Isocitrate dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate into α α -KG) using NADP as co-substrate. Mutations in IDH1 are specific to Arg132 (R132) and endow them with the function of generating 2-hydroxyglutarate (2HG) instead of α-KG. This product alters gene transcription through effects on DNA and histone methylation. Several IDH1 mutations exist, including R132H, R132C, R132S, R132G and R132L. Each may result in different tumor type with varied malignant progression. The most frequent known mutation (>90%) is the alteration of arginine to histidine (R132H). Hence, antibodies that recognize the IDH1R132H mutation can be useful for the diagnosis of mutation-bearing tumors like gliomas.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Bin Sheng Wong et al.
Nature biomedical engineering, 5(1), 26-40 (2020-09-30)
Clinical scores, molecular markers and cellular phenotypes have been used to predict the clinical outcomes of patients with glioblastoma. However, their clinical use has been hampered by confounders such as patient co-morbidities, by the tumoral heterogeneity of molecular and cellular
Peroxisomal localization and function of NADP+-specific isocitrate dehydrogenases in yeast
Lu Q and McAlister-H L.
Archives of Biochemistry and Biophysics, 493(2), 125-134 (2010)
Expression of Idh1R132H in the murine subventricular zone stem cell niche recapitulates features of early gliomagenesis
Bardella C, et al.
Cancer Cell, 30(4), 578-594 (2016)
IDH1R132H decreases the proliferation of U87 glioma cells through upregulation of microRNA-128a
Nie Q M, et al.
Molecular Medicine Reports, 12(5), 6695-6701 (2015)
Yongying Gao et al.
Molecular medicine reports, 23(5) (2021-03-25)
Isocitrate dehydrogenase1 (IDH1) mutation is the most important genetic change in glioma. The most common IDH1 mutation results in the amino acid substitution of arginine 132 (Arg/R132), which is located at the active site of the enzyme. IDH1 Arg132His (R132H)
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