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Key Documents

N5408

Sigma-Aldrich

Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse

clone ME20.4, ascites fluid

Synonym(s):

Anti-NGFR p75

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About This Item

MDL number:
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

ME20.4, monoclonal

mol wt

antigen 75 kDa

contains

15 mM sodium azide

species reactivity

racoon, monkey, pig, human, feline

should not react with

mouse, chicken, rat

technique(s)

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tongue
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
neutralization: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NGFR(4804)

Related Categories

General description

Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) (mouse IgG1 isotype) is derived from the ME20.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a human melanoma cell line WM245. The human NGFR p75 has a hydrophobic signal sequence, a single N-linked glycosylation site, four cysteine-rich repeat units of approximately 40 amino acids in the extracellular domain, a serine- and threonine-rich region which might be O-linked glycosylated, a single transmembrane domain, and a 155-amino acid cytoplasmic domain.
Recognizes the low affinity human NGFR p75 (also known as gp75NGFR , p75NGFR and p75NTR). The epitope recognized by the antibody exists within the third or fourth cysteine-rich repeats of the NGFR molecule.

Specificity

Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) recognizes the low affinity NGFR p75 of human origin (75 kDa), also known as gp75NGFR, p75NGFR and p75NTR .The epitope recognized by the antibody is located within the third or fourth cysteine-rich repeats of the NGFR molecule. Cross-reactivity has been observed with human, monkey (marmoset), pig, cat and raccoon , but not with rat, chicken or mouse NGFR.

Immunogen

human melanoma cell line WM245.

Application

Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse has been used in immunoblotting. to added in CB.
Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) may be used:
  • for enzyme linked immune sorbent assay (ELISA)
  • immunoprecipitation, immunoblotting and immunohistochemistry
  • for immunocytochemistry (acetone- or formalin-fixed, Bouin′s solution - or formalin- fixed paraffin-embedded)
  • for electron- microscopy radioimmunoassay (RIA) (Kd of 7x10-10 M)
  • flow cytometry
  • for the inhibition of binding of NGF to the receptor
  • for rossette assay
  • as a cholinergic immunotoxin

Biochem/physiol Actions

Nerve Growth Factor Receptor (NGFR) p75 enhances the NGF binding affinity of the proto-oncogene product p140trk and also may modulate the kinase activity of p140trk and play a role in signal transduction. Specifically, neurotrophin binding to NGFR p75 stimulates generation of ceramide, activation and translocation of nuclear factor- κ (NF-κB) to the nucleus, and enhancement of Jun kinase (JNK) activity.
Neurotrophins (NT) are growth factors that are essential for the development and maintenance of the nervous system. One of the receptors that mediate the effects of NTs is the Nerve Growth Factor Receptor/TNFRSF16 (p75NGFR, p75NTR). NGFR mediates the effects of NGF that are important in the survival and differentiation of neurons and neural crest-derived cells, during embryonic and early postnatal development. Expression of NGFR has been associated with favorable prognosis and survival in breast and pancreatic cancers.

Physical form

The product is supplied as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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High affinity nerve growth factor binding displays a faster rate of association than p140trk binding. Implications for multi-subunit polypeptide receptors.
Mahadeo D, et al.
The Journal of Biological Chemistry, 269(9), 6884-6891 (1994)
Barbara Kofler et al.
International journal of molecular sciences, 19(4) (2018-04-13)
Photodynamic therapy (PDT) is suggested to have an impact on the treatment of early stage head and neck cancers (HNSCC). We investigated the effect of PDT with methylene blue (MB) and a diode laser (660 nm) as the laser source
Expression and structure of the human NGF receptor
Johnson D, et al.
Cell, 47(4), 545-554 (1986)
Common olfactory ensheathing glial markers in the developing human olfactory system
Oprych K, et al.
Brain Structure &Amp; Function, 222, 1877-1895 (2017)
Nerve growth factor regulates dopamine D(2) receptor expression in prolactinoma cell lines via p75(NGFR)-mediated activation of nuclear factor-kappaB
Fiorentini C, et al.
Molecular Endocrinology, 16(2), 353-366 (2002)

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