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MAK134

Sigma-Aldrich

Ketone Body Assay

sufficient for 200 tests (UV)

Synonym(s):

Ketone Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 200 tests (UV)

feature

This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; neonatal diseases; pediatric diseases; cancer

storage temp.

−20°C

Related Categories

General description

Ketone bodies, 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc), are produced in the liver primarily from oxidation of fatty acids,[1] and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypoglycemia, growth hormone deficiency, alcohol or salicylate intoxication, and inborn errors of metabolism.[2][3]

Application

Ketone Body Assay has been used to measure the release of ketone bodies in the human liver cancer cell line HepG2 culture medium.[4]

Features and Benefits

Compatible with high-throughput handling systems

Suitability

Suitable for the detection of ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) in serum, plasma, urine, and other biological samples.

Principle

In this kit, AcAc and BOH levels are determined using an enzymatic assay based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance measured at 340 nm is directly related to either the AcAc and BOH concentrations.

Storage Class Code

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
134CF01A08
134CF01A08
134CE06A28
134CE06A28
134CD11A21

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Role of β-hydroxybutyrate, its polymer poly-β-hydroxybutyrate and inorganic polyphosphate in mammalian health and disease.
Dedkova E N and Lothar A B
Frontiers in Physiology, 5, 260-260 (2014)
Zachary M Augur et al.
Frontiers in nutrition, 5, 91-91 (2018-10-24)
Introduction: Temozolomide (TMZ) is part of the standard of care for treating glioblastoma multiforme (GBM), an aggressive primary brain tumor. New approaches are needed to enhance therapeutic efficacy and reduce toxicity. GBM tumor cells are dependent on glucose and glutamine
Hyperketonemia and ketosis increase the risk of complications in type 1 diabetes.
Kanikarla M, et al.
Free Radical Biology & Medicine, 95, 268-277 (2016)
Kim J Shimy et al.
Journal of the Endocrine Society, 4(7), bvaa062-bvaa062 (2020-07-16)
According to the carbohydrate-insulin model of obesity, an elevated insulin-to-glucagon ratio in response to a high-carbohydrate diet directs metabolic fuels toward storage, resulting in lower circulating energy. To determine differences in total circulating energy post-meal related to dietary carbohydrate. Ancillary
Role of beta-hydroxybutyric acid in diabetic ketoacidosis: A review.
Stojanovic, et al.
The Canadian Veterinary Journal. La Revue Veterinaire Canadienne, 52(4), 426-426 (2011)
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Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. is this kit compatible for mouse serum/plasma?

    1 answer

    1. Yes, this kit have been used successfully in mouse, rat, bovine, and human samples.

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  4. What is the source of the HBDH enzyme?

    1 answer

    1. The species and source of the HBDH enzyme (3-hydroxybutyrate dehydrogenase) in the assay is native Acaligenes faecalis.

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  5. What is the sensitivity range of this kit?

    1 answer

    1. The kit is sensitive and accurate, utilizing a 10 μL sample. It offers a linear detection range of 0.12 to 8 mM for each ketone body in a 96-well plate assay.

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  6. Is whole blood or plasma collected in EDTA suitable for use with MAK134 Ketone Body Assay?

    1 answer

    1. Certainly, whole blood or plasma collected in EDTA is appropriate for use with this kit. During the development of the kit, these sample types were tested as they are commonly used by researchers. And also AcAc (Acetoacetic acid) is quite unstable, so it is advised using fresh samples or ones that have been frozen within the month.

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  7. What is the range of quantification for both analytes in this assay kit?

    1 answer

    1. This kit has a linear detection range of 0.12 to 8 mM for each ketone body in a 96-well plate assay.

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