Although the activity of D4943 Dipeptidyl Peptidase IV human is not determined using the kit, the product should be suitable for use with the kit for screening DPP4 assay inhibitors.
MAK088
DPP4 Activity Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
CD26 Activity Assay Kit, Deaminase Complexing Protein 2 Activity Assay Kit, Dipeptidyl Peptidase-4 Activity Assay Kit
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About This Item
Pricing and availability is not currently available.
Recommended Products
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
storage temp.
−20°C
Gene Information
human ... DPP4(1803)
mouse ... DPP4(13482)
rat ... DPP4(25253)
General description
Dipeptidyl peptidase-4 (DPP4), also known as CD26 and adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-Proline and X-Alanine residues from the N-termini of polypeptides.[1] DPP4 is a transmembrane glycoprotein whose activity regulates the bioactivity of multiple peptides such as growth factors, chemokines, and neuropeptides. DPP4 plays a major role in glucose metabolism via the regulation of glucagon-like peptide-1 and inhibitors of DPP4 are commonly used for the treatment of type 2 diabetes. DPP4 also plays an important role in immune regulation and may play a role in tumor suppression. Deletion of the DPP4 gene affects carbohydrate metabolism which in turn leads to mental retardation and hypotonia. Abnormal expression of the protein is observed during liver regeneration, cirrhosis and liver tumorigenesis.[2][3]
Application
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the measurement of dipeptidyl peptidase-4 (DPP4) in a variety of samples including tissue, cells and serum
Principle
The DPP4 Activity Assay kit provides a simple and direct procedure for measuring DPP4 activity in a variety of samples. In this assay, DPP4 cleaves a non-fluorescent substrate, H-Gly-Pro-AMC, to release a fluorescent product, 7-Amino-4-Methyl Coumarin (AMC) (λex = 360/λem = 460 nm). One unit of DPP4 is the amount of enzyme that will hydrolyze the DPP4 substrate to yield 1.0 μmole of AMC per minute at 37 °C.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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Because the positive control in MAK088 DPP4 activity kit is a recombinant human DPP4, could I order extra "D4943 Dipeptidyl Peptidase IV human" so that I can use the kit as an inhibitor screening kit? Thank you in advance for your reply.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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Which is th origin of the DPP4 positive control included in the kit, is it human or porcine?
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The positive control in this kit is a recombinant human DPP4.
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Hi, I purchased DPP4 Kit (MAK088) for testing pulse flour samples. The DPP4 assay buffer is limited in amount for hydrating pulse flour samples. Could I use 0.1M, pH8 Tris-HCl buffer instead? and what is the major component of your DPP4 assay buffer?
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The assay buffer supplied in Kit MAK088 is optimized for the reactions they are designed for. It is recommended to use the buffers in the kit. The components within the buffer are considered confidential and cannot be disclosed.
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Pourrait-on savoir les composants de ce MAK088-1KIT s'il vous plait?
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The DPP4 Activity Assay Kit contains:
DPP4 Assay Buffer 25 mL
DPP4 Substrate, H-Gly-Pro-AMC 0.2 mL
DPP4 Positive Control 20 mL
AMC Standard, 1 mM 0.1 mL
DPP4 Inhibitor, Sitagliptin 1 mLHelpful?
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