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M8537

Sigma-Aldrich

MCDB 131 Medium

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

concentration

11.6 g/L

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

General description

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). 

Application

MCDB-131 Medium has been used:

  • as a component of the basal medium to culture bovine retinal endothelial cells
  • to culture bovine lung microvascular endothelial cells
  • for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells

Quantity

Formulated to contain 11.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.18 g/L sodium bicarbonate.

also commonly purchased with this product

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supplement

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Description
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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Abdul Jaleel et al.
Molecular and cellular biochemistry, 451(1-2), 1-10 (2018-06-24)
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G S Ajithkumar et al.
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Endocardial endothelial cells (EECs), when compared with endothelial cells of arteries and veins, possess higher resistance to apoptosis-inducing anticancer agents. The mechanism of this resistance property is unknown. We have investigated the molecular mechanism, which contributes to increased cell survival
Sandra S Hammer et al.
Diabetologia, 64(7), 1674-1689 (2021-03-27)
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Thomas P Burke et al.
eLife, 10 (2021-08-24)
Arthropod-borne rickettsial pathogens cause mild and severe human disease worldwide. The tick-borne pathogen Rickettsia parkeri elicits skin lesions (eschars) and disseminated disease in humans; however, inbred mice are generally resistant to infection. We report that intradermal infection of mice lacking
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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