I0138
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in mouse
clone RG-16, ascites fluid
Synonym(s):
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
RG-16, monoclonal
contains
15 mM sodium azide
should not react with
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
technique(s)
indirect ELISA: 1:10,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are the members of the eponymous immunoglobulin super-family (IgSF). Immunoglobulins are heterodimeric proteins characterized with two heavy (H) and two light (L) chains. Based on heavy chain C domains immunoglobulins are classified in to five main classes namely IgM, IgG, IgA, IgD, and IgE isotypes.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
Specificity
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Application
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA), immunoblots, dot blots and immunohistochemistry.
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Biochem/physiol Actions
Immunoglobulins acts as a cell-surface receptor for antigen that allows cell signaling and cell activation. In addition, it also acts as a soluble effector molecule that can recognize, bind and neutralize antigens. IgG contributes to immune response including neutralization of toxins and viruses. IgM provides first line of defense and is involved in immunoregulation. IgA protects mucous membranes against toxins, virus and bacteria by direct neutralization or by prevention of binding to the mucosal membranes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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