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BPQ24

Sigma-Aldrich

BioPORTER® QuikEase Protein Delivery Kit

for intracellular delivery

Synonym(s):

BioPORTER Kit, Protein Delivery Kit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

Quality Level

technique(s)

transduction: suitable

shipped in

dry ice

storage temp.

−20°C

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General description

Contains 24 individual tubes of BioPORTER reagent

Application

BioPORTER® QuikEase Protein Delivery Kit has been used to deliver active granzyme B tagged with FITC into mouse neuroblastoma cell lines. Protein concentrations used for delivery were 6μg/ml which when stained for FITC-GrB yielded a 90% uptake rate.
BioPORTER® QuikEase Protein Delivery Kit has been used:
  • for the delivery of single-chain antibody variable fragment (scFv) into HeLa cells
  • for the transduction of QBI-293 cells with human Tau protein
  • for the delivery of human granzyme B (GrB) in human cervical cancer cells
  • for the delivery of epidermal fatty acid-binding protein (E-FABP) into nerve growth factor-differentiated PC12 cells (NGFDPC12)

The BioPORTER® QuikEase Protein Delivery Kit is the latest innovation in macromolecule delivery technology. It is a unique lipid-based formulation that allows for the intracellular delivery of proteins, peptides, and bioactive molecules such as enzymes and antibodies, into a broad range of cell types. The BioPORTER QuikEase kits contain individual tubes of the BioPORTER Reagent in a convenient, ready-to-use, single reaction format. Each tube contains sufficient material of the lyophilized BioPORTER Reagent to perform 1 reaction in a 6-well plate (or 35 mm dish), 4 reactions in a 24-well plate (or 16 mm wells), and 10 reactions in a 96-well plate.

Legal Information

BioPorter is a registered trademark of Gene Therapy Systems, Inc.
QuikEase is a trademark of Gene Therapy Systems, Inc.

Kit Components Also Available Separately

Product No.
Description
SDS

  • BioPorter® Reagent Coated Tubes

  • FITC-Antibody Control

  • β-Galactosidase Control

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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E. coli expression of a soluble, active single-chain antibody variable fragment containing a nuclear localization signal
Xiong H, et al.
Protein Expression and Purification, 66(2), 172-180 (2009)
Cancer vaccination drives Nanog-dependent evolution of tumor cells towards an immune-resistant and stem-like phenotype
Noh KH, et al.
Cancer Research, canres-ca3758 (2012)
Seeding of normal Tau by pathological Tau conformers drives pathogenesis of Alzheimer-like tangles
Guo JL and Lee VMY
The Journal of Biological Chemistry, 286(17), 15317-15331 (2011)
Xianzhi Jiang et al.
Journal of virology, 85(5), 2325-2332 (2010-12-24)
The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) is the only HSV-1 gene transcript abundantly expressed throughout latency. LAT null mutants have a significantly reduced reactivation phenotype. LAT's antiapoptosis activity is the major LAT factor involved in supporting
Jason C Sang et al.
Communications biology, 4(1), 613-613 (2021-05-23)
Aggregation of α-synuclein (α-syn) is closely linked to Parkinson's disease (PD) and the related synucleinopathies. Aggregates spread through the brain during the progression of PD, but the mechanism by which this occurs is still not known. One possibility is a

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