SCC464

sLT-RMS Spontaneous Lung-tropic RET Melanoma-sorted Mouse Cell Line

Mouse

• Synonym(s): Q61R NRAS melanoma cell line, SK-MEL-147, human melanoma cell line, human melanoma cells, metastatic melanoma cell line
• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• product name: sLT-RMS Spontaneous Lung-tropic RET Melanoma-sorted Mouse Cell Line,
• biological source: mouse
• Quality Level: 100
• packaging: vial of ≥1X10⁶ vial
• manufacturer/tradename: Millipore
• technique(s): cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Description

General description

The mouse spontaneous lung-tropic Ret-melanoma (sLT-RMS) cell line, was isolated from the lung metastasis following the subdermal injection of RMS cells. Like the parental RMS line (SCC462), sLT-RMS cells express mCherry and are puromycin resistant. sLT-RMS cells express the common melanoma markers, PMEL and Melan-A, along with the chemokine receptor CXCR3.

Application

• Each vial contains >1X10^? viable cells.
• sLT-RMS cells are verified to be of mouse origin and negative for human, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells tested negative for infectious diseases against a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma.

Features and Benefits

SCC464, the sLT-RMS spontaneous lung-tropic RET melanoma-sorted mouse cell line, expresses mCherry and its CXCR3 expression may be critical for melanoma metastasis.

Target description

The RET oncogene was originally created by an accidental in vivo recombination of two human genes, RET and RFP (the RET-finger protein, known today as TRIM27), during a transformation of NIH3T3 cells with human lymphoma DNA (Takahashi 1985). The founder R

Storage and Stability

sLT-RMS cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact l

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable