The NHP Cytokine Panel 1 (PRCYTOMAG-40K) kit was modified such that the TNF-a standard lot is value assigned. The first standard lot with this change was SC840-22K. This adjustment should not cause QCs to read out of range as it is still calibrated to the gold standard. The raw material used for the TNF-a standard has not changed. TNFα was identified as an analyte that would benefit from a more robust production process.
PRCYTOMAG-40K
MILLIPLEX® Non-Human Primate Cytokine Magnetic Bead Panel - Immunology Multiplex Assay
Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Synonym(s):
Luminex® non-human primate cytokine immunoassay, Millipore non-human primate cytokine panel, Monkey cytokine multiplex kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
To order a Milliplex® kit, please search for your analyte of interest.
Recommended Products
Quality Level
species reactivity
nonhuman primates
manufacturer/tradename
Milliplex®
assay range
accuracy: 70-101%
standard curve range: 12.2-50,000 pg/mL
(IL-10, IL-18)
standard curve range: 2.4-10,000 pg/mL
standard curve range: 4.9-20,000 pg/mL
(IL-4)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
ambient
General description
The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition, cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis, and cancer.
The MILLIPLEX® Non-Human Primate Cytokine panel is to be used for the simultaneous quantification of any or all of the following in tissue/cell lysate and culture supernatant samples and serum or plasma samples: G-CSF, GM-CSF, IFNγ, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL8, IL-10, IL-12/23(p40), IL-13, IL-15, IL-17A, MCP-1, MIP-1β, MIP-1α, sD40L, TGF-α, TNF-α, VEGF, and IL-18.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
The MILLIPLEX® Non-Human Primate Cytokine panel is to be used for the simultaneous quantification of any or all of the following in tissue/cell lysate and culture supernatant samples and serum or plasma samples: G-CSF, GM-CSF, IFNγ, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL8, IL-10, IL-12/23(p40), IL-13, IL-15, IL-17A, MCP-1, MIP-1β, MIP-1α, sD40L, TGF-α, TNF-α, VEGF, and IL-18.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
Specificity
Cross Reactivty
The multiplexed assay panel has been tested with samples from multiple non-human
primate species, please see protocol for details.
The multiplexed assay panel has been tested with samples from multiple non-human
primate species, please see protocol for details.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
- Analytes: G-CSF, GM-CSF, IFN-γ, IL-1RA, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12/23 (p40), IL-13, IL-15, IL-17, IL-18, MCP-1, MIP-1α, MIP-1β, sCD40L, TGF-α, TNF-α, VEGF
- Recommended Sample type: Serum, plasma, and cell culture
- Recommended Sample dilution: Neat
- Assay Run Time: Overnight or one day. For best results, an overnight incubation is recommended
- Research Category: Inflammation & Immunology
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
Everything you need in a single kit.
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Other Notes
Sensitivity: Refer to kit protocol for sensitivities for individual cytokines/chemokines
Standard Curve Range: Refer to QC analysis sheet for exact concentration (sCD40L, TNFα)
Standard Curve Range: Refer to QC analysis sheet for exact concentration (sCD40L, TNFα)
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
G-CSF, GM-CSF, IFN-γ, IL-1ra, IL-1ß, IL-2, IL-5, IL-6, IL-8, IL-12/23 (p40), IL-13, IL-15, IL-17, MCP-1, MIP-1α, MIP-1ß, TGF-α, VEGF
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Johannes Lutz et al.
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Tetsu Oura et al.
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We have previously reported successful induction of transient mixed chimerism and long-term acceptance of renal allografts in MHC mismatched nonhuman primates. In this study, we attempted to extend this tolerance induction approach to islet allografts. A total of eight recipients
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Whitney A Nichols et al.
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Tianhong Xie et al.
Signal transduction and targeted therapy, 7(1), 61-61 (2022-02-27)
Variants are globally emerging very quickly following pandemic prototypic SARS-CoV-2. To evaluate the cross-protection of prototypic SARS-CoV-2 vaccine against its variants, we vaccinated rhesus monkeys with three doses of prototypic SARS-CoV-2 inactivated vaccine, followed by challenging with emerging SARS-CoV-2 variants
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Has the concentration of the TNFa standard in the NHP Cytokine Milliplex kit changed? It was previously 10,000 pg/ml but is now listed on the QC Analysis Sheet and changes with each lot.
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Could you provide information about the specificity and cross-reactivity between IL-1b and IL-18 for the antibodies in kit PRCYTOMAG-40K, which is used for detecting monkey cytokines? There is concern that we may not be able to measure both molecules simultaneously.
1 answer-
Individual standards in the kit were tested using multiplexed beads. It was observed that both IL-1b against IL-18 standards and IL-18 against IL-1b standards showed levels below background.
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Does the IL-1B antibodies in the NHP cytokine panel (cat # PRCYTOMAG-40K) measure total IL-1b or the monomeric/bioactive IL-1b?
1 answer-
The IL-1B antibodies included in the NHP cytokine panel (cat # PRCYTOMAG-40K) have not been specifically tested for their ability to measure the active form only. Based on available antibody information, it is known that they can detect both natural and recombinant forms of IL-1B and that they are monoclonal antibodies. However, additional specificity information is not available.
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Do you know what the MAP in the Millipore kits stands for?
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xMAP is a term developed by Luminex. It stands for Multi-Analyte Profiling. The 'x' represents the number of analytes being tested.
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What is the reason for the much lower median readings on the standards in my Milliplex assay after accidentally washing off the detection antibody before adding SAPE?
1 answer-
Lower signal is expected when the detection antibody is washed off and not incubated with the SAPE. SAPE has multiple binding sites, and when incubated with the detection antibodies, it leads to the formation of larger complexes, resulting in an increased signal.
The drop in signal should be equal across the entire plate, and if the quality control results are within the range, the calculated sample results should be acceptable.
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