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AP192C

Sigma-Aldrich

Donkey Anti-Mouse IgG Antibody, Cy3 conjugate, Species Adsorbed

Chemicon®, from donkey

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

donkey

Quality Level

conjugate

CY3 conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Specific for mouse IgG, heavy and light chain.

Application

Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
This Donkey anti-Mouse IgG Antibody, Cy3 conjugate, Species Adsorbed is validated for use in IF for the detection of Donkey Mouse IgG.

Physical form

ImmunoAffinity Purified
Lyophilized. Buffer = 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

Storage and Stability

Store lyophilized material at 2–8°C for up to 1 year from date of shipment. After reconstitution this product is stable for several weeks at 2–8°C as an undiluted liquid. DO NOT FREEZE. Store away from light. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade or better) to make a final concentration of 50% glycerol and store at 2–8°C for up to 6 months. Avoid repeated warming/cooling cycles. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.

Other Notes

Concentration: variable

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neuroprotective effect of the endogenous neural peptide apelin in cultured mouse cortical neurons.
Zeng, XJ; Yu, SP; Zhang, L; Wei, L
Experimental Cell Research null
M T Klein et al.
British journal of pharmacology, 166(4), 1290-1302 (2012-01-21)
The 5-ht(1E) receptor is highly expressed in the human brain and its structure is conserved in humans, suggesting an important physiological role for 5-ht(1E) receptors. However, neither the function nor the distribution of this receptor has been characterized in the
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To define a genetic network that regulates development of the pancreas, we used high-density microarray (Affymetrix) to generate transcriptional profiles of rat pancreas from five biologically significant stages of development: embryonic day 12.5 (E12.5), E15.5, E18.5, postnatal day 0 (P0)
Xiao-Yan Liu et al.
World journal of gastroenterology, 15(14), 1738-1743 (2009-04-11)
To investigate the expression of alpha-fetoprotein (AFP), a cancer-associated fetal glycoprotein, and its involvement during rat colon development. Colons from Sprague-Dawley rat fetuses, young and adult (8 wk old) animals were used in this study. Expression levels of AFP in
Lijie Liu et al.
Development, growth & differentiation, 49(8), 669-681 (2007-09-21)
To identify proteins involved in pancreatic development, we used a differential proteomics approach by comparing pancreatic extracts from four biologically significant stages of development: embryonic day (E) 15.5, E18.5, postnatal (P) days 0 and adult. By two-dimensional gel electrophoresis (2D-E)

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