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574793

Sigma-Aldrich

TE Buffer, 100X, Molecular Biology Grade

A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.

Synonym(s):

Tris-EDTA Buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25
Pricing and availability is not currently available.

grade

Molecular Biology

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

General description

A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.
Tris-ethylenediaminetetraacetic (TE) buffer is a common buffer used in molecular biology to maintain the integrity of nucleic acid during handling and storage.[1]

Application

TE Buffer, 100X, Molecular Biology Grade – Calbiochem has been used to dilute DNA stock solutions.[2] It has also been used to dilute thymidylate synthase (thyA) culture for colony polymerase chain reaction (PCR).[3]

Warning

Toxicity: Irritant (B)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ping Song et al.
Nature biomedical engineering, 5(7), 690-701 (2021-05-05)
DNA sequence variants with allele fractions below 1% are difficult to detect and quantify by sequencing owing to intrinsic errors in sequencing-by-synthesis methods. Although molecular-identifier barcodes can detect mutations with a variant-allele frequency (VAF) as low as 0.1% using next-generation
Victor Chen et al.
Applied and environmental microbiology, 87(18), e0084421-e0084421 (2021-07-08)
Enterococcus faecium is a ubiquitous Gram-positive bacterium that has been recovered from the environment, food, and microbiota of mammals. Commensal strains of E. faecium can confer beneficial effects on host physiology and immunity, but antibiotic usage has afforded antibiotic-resistant and
Jason W Johnston et al.
Plant physiology and biochemistry : PPB, 43(9), 844-853 (2005-11-18)
HPLC analysis of nucleosides is important for determining total DNA methylation in plants and can be used to help characterise epigenetic changes during stress, growth and development. This is of particular interest for in vitro plant cultures as they are

Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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