14-450-M

Cdk1/cyclin B Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.

• Synonym(s): Active Cdk1/cyclin B, Cdk1 Protein
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.26

Properties

• biological source: human
• Quality Level: 100
• form: liquid
• shelf life: 6 mo.
• mol wt: M_w 35 kDa (cdk1); M_w 75 kDa (cyclin B)
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• solubility: water: soluble
• NCBI accession no.: NM_001786.2; NM_033379.2
• UniProt accession no.: P06493
• storage temp.: −70°C
• Gene Information: human ... CCNB1(891) , CDK2(891)

Description

General description

Research area: Apoptosis
Cyclin dependent kinase 1 (CDK1), a type of serine/threonine kinase, is a catalytic subunit of the M phase-promoting factor (MPF) complex.

Biochem/physiol Actions

Cyclin B protein regulates mitosis in all eukaryotes. Cyclin B binds to cyclin dependent kinase 1 (CDK1), forming a complex that triggers the start of mitosis by phosphorylating specific proteins. Cyclin B regulates the activation, subcellular localization, and target selection of CDK1. Its destruction is essential for the completion of mitosis. Overexpression of human cyclin B1 has been linked to aggressive tumor behavior and is frequently observed in various cancers.
CDK1 plays a key role in driving the M-phase in both meiosis and mitosis. It becomes highly active at the start of the M-phase and becomes inactive at the end. CDK1 controls various important events in the cell, such as DNA replication, segregation, and repair, as well as mRNA transcription and cell morphogenesis. Additionally, it regulates ribosome assembly by targeting specific ribosomal proteins.

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of the Histone H1 substrate

Physical form

Purified using Ni²⁺/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose^™ and Ni2+/NTA-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

Sepharose is a trademark of Cytiva

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.