Product 1326570001 is approved for In Vitro Diagnostic Use, specifically for staining tissues embedded in paraffin. The expectation is that a light or brightfield microscope is used to evaluate the staining. However, no applications have been developed for using the solution for TEM sections embedded in epoxy resin. When performing TEM, the staining times will likely be prolonged, especially if the resin cannot be removed.
1.32657
Alcian blue staining kit, pH 2.5
for the detection of acidic mucosubstance for microscopy
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About This Item
UNSPSC Code:
41116130
NACRES:
NA.41
Pricing and availability is not currently available.
Recommended Products
Quality Level
IVD
for in vitro diagnostic use
pH
2.5
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
General description
Alcian blue pH 2.5 - for the detection of acidic mucosubstances - for microscopy, is a ready-to-use staining kit used to stain acidic mucopolysaccharides in human-histological specimen materials, for example histological sections of e. g. the intestine or stomach. Acidic mucosubstances are selectively stained in the tissue, after which the specimen is counterstained with nuclear fast red. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. The package is sufficient for approx. 2500 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Application
For the detection of acidic mucosubstances.
For Microscopy.
For Microscopy.
Analysis Note
Nuclei: red
Acidic mucosubstances: light blue
Acidic mucosubstances: light blue
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Can the kit 1326570001 be used for TEM grids with sections at ~70 nm thick? The sections on the TEM grids are epoxy resin sections.
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