The concentration of the Gram's Safranine solution is considered proprietary since the concentration is not listed in either the User's Guide or the Safety Data Sheet. Most textbook formulations for Grams Safranin will list the concentration as somewhere between 0.25% to 0.6% dye content.
1.09217
Gram′s safranine solution
for the Gram staining method
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About This Item
UNSPSC Code:
41116130
NACRES:
NA.74
Pricing and availability is not currently available.
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Quality Level
form
liquid
IVD
for in vitro diagnostic use
technique(s)
microbe id | staining: suitable
transition temp
flash point 49 °C
density
0.98 g/cm3 at 20 °C
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
General description
This ready-to-use Gram′s safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution and appear pink to red.
A bottle with 500 ml Gram′s safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
A bottle with 500 ml Gram′s safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
120.2 °F
Flash Point(C)
49 °C
Certificates of Analysis (COA)
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I have Gram's safranin solution for microscopy MERCK want to know about the conc. Of this product.
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