06-340
Anti-Myc Antibody
Upstate®, from rabbit
Synonym(s):
Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, Xenopus, avian
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... MYC(4609)
General description
c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression.
Specificity
Recognizes Myc isoforms Mr 50-67kDa.
Will also cross-react with avian and Xenopus.
Immunogen
Bacterially expressed fusion-protein corresponding to the full-length human Myc.
Application
Immunoprecipitation:
4 µg of a previous antibody immuno-precipitated Myc as shown by an independent laboratory.
Immunocytochemistry:
This antibody has been used for immunocytochemistry by an independent laboratory. (Ohtsuka, T., 1998)
4 µg of a previous antibody immuno-precipitated Myc as shown by an independent laboratory.
Immunocytochemistry:
This antibody has been used for immunocytochemistry by an independent laboratory. (Ohtsuka, T., 1998)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-Myc Antibody (rabbit polyclonal antibody) validated in ChIP, ICC, IP, WB to detect Myc also known as Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein.
Quality
Routinely evaluated by western blot on A431 lysates from EGF-stimulated human A431 cells, human A431 cells and HeLa nuclear extract.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Myc in A431 lysates from EGF-stimulated human A431 cells. 0.5-2 µg/mL of a previous lot detected Myc in RIPA lysates from human A431 cells and HeLa nuclear extract.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Myc in A431 lysates from EGF-stimulated human A431 cells. 0.5-2 µg/mL of a previous lot detected Myc in RIPA lysates from human A431 cells and HeLa nuclear extract.
Target description
67 kDa
Physical form
Format: Purified
Immunoaffinity Chromatography
Purified rabbit polyclonal IgG in buffer containing PBS, 0.05% sodium azide with 2 mg/mL BSA. Frozen solution.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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