816260

TSKgel^® Phenyl-5PW RP (13 µm) HPLC Columns

L x I.D. 15 cm × 21.5 mm, phenyl phase

• Synonym(s): TSKgel^® Reversed Phase HPLC Column (Polymer-based)
• UNSPSC Code: 41115711
• eCl@ss: 32110501

Properties

• product name: TSKgel^® Phenyl-5PW RP HPLC Column, phase phenyl, L × I.D. 15 cm × 21.5 mm, 13 μm particle size
• material: stainless steel hardware
• product line: TSKgel^®
• manufacturer/tradename: Tosoh Phenyl-5PW, 16260
• extent of labeling: 1 meq/mL ligand density (estimate)
• parameter: no limitation salt concentration; 0-100% organic solvent; 30 bar max. pressure; 4-60 °C temperature; 8 mL/min flow rate
• technique(s): HPLC: suitable
• L × I.D.: 15 cm × 21.5 mm
• matrix: polymer particle platform
• matrix active group: phenyl phase
• particle size: 13 μm
• pore size: 1000 Å
• operating pH: 2-12
• separation technique: reversed phase

Description

General description

TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients. The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel Phenyl-5PW RP can withstand rigorous cleaning protocols using either acid or base. The 1000Å pore size accommodates globular protein samples up to 1,000,000 Da.

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

• Octadecyl-2PW particles have 125Å pores
• Octadecyl-4PW particles have 500Å pores
• Phenyl-5PW RP particles have 1000Å pores
• Octadecyl-NPR particles are nonporous

Physical form

Shipped in 30% methanol - 70% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation