S4072

Anti-Sirt3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-SIR2L3, Anti-Sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• MDL number: MFCD07784765
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~28 kDa
• species reactivity: human
• technique(s): indirect immunofluorescence: suitable; western blot: 2.5-5 μg/mL using human HepG2 cells
• UniProt accession no.: Q9NTG7
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SIRT3(23410)

Description

General description

Sirtuin 3 (Sirt3) is an NAD-dependent deacetylase localized to the mitochondrial matrix. SIRT3 encodes a 44 kDa full length form and a N-terminal 142 amino acids truncated form. It is mapped to human chromosome 11p15.5.

Immunogen

synthetic peptide corresponding to amino acids 384-399 of human SIRT3, conjugated to KLH via an N-terminal cysteine residue.

Application

Anti-Sirt3 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Sirtuin 3 (Sirt3) is mainly a nuclear protein that transfers to the mitochondria during cellular stress. In humans, Sirt3 deacetylates and activates the mitochondrial enzyme acetyl-CoA synthetase 1 (AceCS2). Sirt3 is implicated in regulating mitochondrial function and thermogenesis in brown adipocytes. Its levels have been shown to correlate with extended life span. Increased levels of Sirt3 were associated with node positive breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves