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P7892

Sigma-Aldrich

Phenyl-Sepharose CL-4B

extent of labeling: ~40 μmol per mL

Synonym(s):

Phenyl-Agarose

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About This Item

MDL number:
MFCD00147908
UNSPSC Code:
41106500
NACRES:
NA.56

form

suspension

extent of labeling

~40 μmol per mL

matrix

agarose, 4% cross-linked

matrix activation

epichlorohydrin

matrix attachment

hydroxyl

matrix spacer

3 atoms

particle size

45-165 μm

capacity

15-20 mg/mL binding capacity (human serum albumin)
 3-5 mg/mL binding capacity (β-lactoglobulin)

storage temp.

2-8°C

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General description

P7892-200ML′s updated product number is GE17-0810-01

Application

Phenyl Sepharose™ is used in protein chromatography, affinity chromatography, hydrophobic interaction media, resins and separation media. Phenyl Sepharose™ has been used in studies that contributed to improving industrial applications in additives in detergents and feed industries. Phenyl Sepharose™ has also been used to study microbial communities inhabiting hypersaline environments.

Physical form

Suspension in 20% ethanol
aqueous ethanol suspension

Legal Information

Sepharose is a trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

96.8 °F

Flash Point(C)

36 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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O Ploux et al.
The Biochemical journal, 283 ( Pt 2), 327-331 (1992-04-15)
The 8-amino-7-oxopelargonate synthase [6-carboxyhexanoyl-CoA:L-alanine carboxyhexanoyltransferase (decarboxylating); EC 2.3.1.47] from Bacillus sphaericus involved in biotin biosynthesis was purified from an Escherichia coli overproducing strain. The purification afforded an electrophoretically homogeneous enzyme with a specific activity of 0.67 unit/mg. The purified enzyme
Sheref S Mansy et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(36), 13351-13355 (2008-09-05)
The earliest cells may have consisted of a self-replicating genetic polymer encapsulated within a self-replicating membrane vesicle. Here, we show that vesicles composed of simple single-chain amphiphiles such as fatty acids, fatty alcohols, and fatty-acid glycerol esters are extremely thermostable
W Kroutil et al.
Journal of biotechnology, 61(2), 143-150 (1998-07-09)
A highly enantioselective, soluble epoxide from Nocardia sp. EH1 was purified to homogeneity via a four-step procedure: (i) hydrophobic interaction chromatography on Phenyl Sepharose CL-4B, (ii) anion exchange chromatography on SOURCE 30Q, followed by (iii) a second hydrophobic interaction chromatography
Purification and properties of hepatic glutamine synthetases from the ureotelic gulf toadfish, <I>Opsanus beta</I>.
Walsh, P.J., et al.
Comp. Biochem. Physiol., B: Comp. Biochem., 115(4), 523-532 (1996)
Sheref S Mansy et al.
Nature, 454(7200), 122-125 (2008-06-06)
Contemporary phospholipid-based cell membranes are formidable barriers to the uptake of polar and charged molecules ranging from metal ions to complex nutrients. Modern cells therefore require sophisticated protein channels and pumps to mediate the exchange of molecules with their environment.
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