KCQS08
KiCqStart® One-Step Probe RT-qPCR ReadyMix™, Low ROX™
with Low ROX for ABI and Stratagene instruments
Synonym(s):
KiCqStart® One Step Probe RT-qPCR Readymix™
About This Item
Recommended Products
form
liquid
usage
sufficient for 500 reactions
feature
dNTPs included
hotstart
storage condition
protect from light
technique(s)
qPCR: suitable
color
light blue
input
purified RNA
compatibility
ABI 7500 Fast
ABI 7500
ABI QuantStudio
ABI ViiA 7
Agilent AriaMx
Douglas Scientific IntelliQube
Qiagen Corbett Rotor-Gene Q
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000
detection method
probe-based
shipped in
dry ice
storage temp.
−20°C
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General description
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is highly resistant to PCR inhibitors. A key component of the ReadyMix™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product.
Application
- Applied Biosystems® 7500
- Applied Biosystems 7500 Fast
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio
- Agilent® AriaMx
- Douglas Scientific IntelliQube
- QIAGEN® Rotor-Gene® Q
- Stratagene Mx3000P®
- Stratagene Mx3005P®
- Stratagene Mx4000™
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Components
packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume
Legal Information
recommended
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
Protocols
KiCqStart One-Step Probe RT-qPCR ReadyMix streamlines RNA template RT-qPCR with high sensitivity.
KiCqStart One-Step Probe RT-qPCR ReadyMix streamlines RNA template RT-qPCR with high sensitivity.
KiCqStart One-Step Probe RT-qPCR ReadyMix streamlines RNA template RT-qPCR with high sensitivity.
KiCqStart One-Step Probe RT-qPCR ReadyMix streamlines RNA template RT-qPCR with high sensitivity.
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