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HPA004935

Sigma-Aldrich

Anti-CLEC4E antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-C-type lectin domain family 4, member E, Anti-CLECSF9, Anti-mincle

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
NACRES:
NA.43

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:50-1:200

immunogen sequence

SCYFFSTDTISWALSLKNCSAMGAHLVVINSQEEQEFLSYKKPKMREFFIGLSDQVVEGQWQWVDGTPLTKSLSFWDVGEPNNIATLEDCATMRDSSNPRQNWNDVTCFLNYFRI

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CLEC4E(26253)

General description

CLEC4E (C-type lectin domain family 4, member E) belongs to C-type lectin receptors, which is a family of pattern recognition receptors. C-type lectin receptor family has more than 1000 members and includes any protein which has one or more C-type lectin-like domain (CLTD). CLEC4E, also called mincle, is a type II transmembrane protein, which is 219 aa long and has a highly conserved CLTD. CLEC4E is located on human chromosome 12 within the natural killer (NK) gene complex, which also includes BDCA-2, DCAR, DCIR, Dectin-2, and Clecsf8. CLEC4E protein has a single transmembrane domain, a short N- terminal cytoplasmic domain and a C-terminal extracellular C-type lectin carbohydrate recognition domain.

Immunogen

C-type lectin domain family 4, member E recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

CLEC4E (C-type lectin domain family 4, member E) recognises the glycolipid trehalose-6,6-dimycolate (TDM, also called cord factor) present in Mycobacterium species, pathogenic fungi Malassezia spp., and spliceosome-associated protein 130 (SAP130), which is an endogenous ligand released during cell necrosis. Upon sensing damaged cells, Mincle induces activated macrophages to produce inflammatory cytokines. It plays an important role in the immunological response against Candida albicans infections in mammals. It is also responsible for the inflammation in lupus nephritis, by interacting with SAP130 present in necrotic cell debris and promoting the production of inflammatory cytokines. CLEC4E, in coordination with CLEC4D, helps to control bacterial growth and hyperinflammation in bacterial pneumonia and chronic lung diseases, by regulating phagocytosis and efferocytosis. It also has functions in or has relevance to diseases such as connective tissue disorder, rheumatic disease, arthritis, skeletal and muscular disorder etc.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST70024

Physical form

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Suppression of Tie-1 in endothelial cells in vitro induces a change in the genome-wide expression profile reflecting an inflammatory function.
Chan B and Sukhatme VP
Febs Letters, 583(6), 1023-1028 (2009)
Mincle, the receptor for mycobacterial cord factor, forms a functional receptor complex with MCL and FcεRI-γ.
Lobato-Pascual A
European Journal of Immunology, 43(12), 3167-3174 (2013)
Anton G Kutikhin et al.
Cancer management and research, 4, 39-53 (2012-03-20)
The group of pattern recognition receptors includes families of Toll-like receptors, NOD-like receptors, C-type lectin receptors, and RIG-I-like receptors. They are key sensors for a number of infectious agents, some of which are oncogenic, and they launch an immune response
Myeloid C-type lectin receptors in pathogen recognition and host defense.
Osorio F and Reis e Sousa C
Immunity, 34(5), 651-664 (2011)
Antibacterial and pro-resolving mechanisms in lung diseases: role of C-type lectin receptors (INM2P.430)
Sharma J
Journal of Immunology, 192(1), 56-13 (2014)

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