This product is sufficient for ≤10 assays. When reconstituted with 10 ml deionized water, each vial will yield a solution with a pH of approximately 7.3, containing the following components: 0.3 mM 4-Hydroxyazobenzene-2-carboxylic acid (HABA), 0.45 mg/ml avidin, 0.3 M sodium chloride (NaCl), 0.01 M N-[2-Hydroxyethyl]piperazine-NN-[2-ethanesulfonic acid] (HEPES), a buffer with a pKa of 7.5, 0.01 M magnesium chloride (MgCl2), and 0.02% sodium azide as a preservative.
H2153
HABA/Avidin Reagent
lyophilized powder
Synonym(s):
HABA Reagent
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.
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form
lyophilized powder
Quality Level
usage
sufficient for ≤10 assays
color
orange
suitability
suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples
storage temp.
2-8°C
General description
4-Hydroxyazobenzene-2-carboxylic acid (HABA) is an avidin-binding dye that can be stoichiometrically displaced by biotin. The subsequent change in absorption at A500 provides a basis for spectrophotometric determination of biotin levels in biological samples.
HABA/Avidin Reagent is specific for chromogenic applications that depend upon quantification of biotin.
Application
HABA/Avidin Reagent is suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples.
Reagent is capable of detecting biotin at μMolar concentrations (i.e., 1 nmole per ml). Typically used to quantitate biotin in biotinylated compounds.
Reconstitution
Prepares a ready-to-use HABA/avidin solution in buffered saline, pH approx. 7.3, with preservative when reconstituted with 10 ml deionized water.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Parameters and conditions of an enzyme based assay for biotin and avidin are presented. Biotinylated glucose-6-phosphate dehydrogenase when complexed with avidin becomes inactivated. Thus it was possible to construct a competitive assay system for biotin. The assay is sensitive between
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I would like to know how many assays can be done with a VIAL KIT and how much material it contains,
1 answer-
Helpful?
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how much HABA is in the vial
1 answer-
When reconstituted to 10mL, the vial will contain 0.3 mM HABA. Based on the molecular weight of HABA, 242.23 gmol, the calculated mass per vial is 726.69 ug. Please see the link below to review additional informaition available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/451/h2153dat.pdfHelpful?
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