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Key Documents

C6039

Sigma-Aldrich

Cell Freezing Medium-Glycerol 1×

sterile-filtered, suitable for cell culture

Synonym(s):

Cell Cryopreservation Solution, Freezing Medium for Cells

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: no
HEPES: no

shipped in

dry ice

storage temp.

−20°C

General description

Cell Freezing Medium-Glycerol 1× is designed to protect and preserve cells during frozen storage. It is recommended for the cryopreservation of cells.

Application

Cell Freezing Medium-Glycerol 1× has been used for cryopreservation of honey bee semen, dispersed blastomeres, and whole blastoderms.

Other Notes

Complete ready-to-use medium. Proprietary formulation containing Minimum Essential Medium, glycerol, calf serum and fetal bovine serum.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Cryopreservation of rainbow trout blastoderms
BaBIak I, et al.
Cybium, 32, 139-141 (2008)
Usage of green coconut water and different tissue culture media for in vitro honey bee semen storage (Apis mellifera; Hymenoptera: Apoidea)
Almeida R, et al.
Interciencia, 27, 317-321 (2002)

Protocols

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

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