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OP44

Sigma-Aldrich

Anti-APC (Ab-1) Mouse mAb (FE9)

liquid, clone FE9, Calbiochem®

Synonym(s):

Anti-Adenomatous Polyposis Coli

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

FE9, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... APC(324)

General description

Anti-APC (Ab-1), mouse monoclonal, clone FE9, recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. It is validated for Western botting.
Protein G purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP40 cells. Recognizes the ~300 kDa APC protein as well as a variety of truncated forms.
Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells.
  • Antibody Target Gene Symbol: APC
  • Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
  • Entrez Gene Name: adenomatous polyposis coli
  • Hu Entrez ID: 324 (Related Antibodies: OP80, ST1150, OP62, OP47L)
  • Mu Entrez ID: 11789
  • Rat Entrez ID: 24205

    • Immunogen

    a synthetic peptide corresponding to the N-terminal 35 amino acids of APC

    Application

    Immunoblotting (1 µg/ml, see comments)

    Packaging

    Please refer to vial label for lot-specific concentration.

    Warning

    Toxicity: Standard Handling (A)

    Physical form

    In 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.

    Analysis Note

    Positive Control
    HCT116 cells for p300, SW480 cells for truncated APC (p147)

    Other Notes

    Koetsier, P. A., et al. 1993. BioTechniques15, 258.
    Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.
    Su, L.-K., et al. 1993. Can. Res.53, 2728.
    Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.
    D′Amico, D., et al. 1992. Cancer Res.52, 1996.
    Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.
    Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.
    Powell, S. M., et al. 1992. Nature359, 235.
    Groden, J., et al. 1991. Cell66, 589.
    Kinzler, K. W., et al. 1991. Science253, 661.
    Nishisho, I., et al. 1991. Science253, 665.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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    Tamar Evron et al.
    Oncogenesis, 10(9), 63-63 (2021-09-24)
    The Wnt signaling pathways play fundamental roles during both development and adult homeostasis. Aberrant activation of the canonical Wnt signal transduction pathway is involved in many diseases including cancer, and is especially implicated in the development and progression of colorectal
    Hideaki Toki et al.
    Cancer science, 104(7), 937-944 (2013-04-05)
    Mutant mouse models are indispensable tools for clarifying the functions of genes and elucidating the underlying pathogenic mechanisms of human diseases. We carried out large-scale mutagenesis using the chemical mutagen N-ethyl-N-nitrosourea. One specific aim of our mutagenesis project was to
    Mireia Menéndez et al.
    Gastroenterology, 134(1), 56-64 (2008-01-02)
    We identified the APC N1026S variant of unknown malignant potential in the adenomatous polyposis coli (APC) gene in a Spanish attenuated familial adenomatous polyposis (AFAP) family. The variant was located in the first of the 4 highly conserved 15-amino acid
    Dipon Das et al.
    DNA repair, 24, 15-25 (2014-12-03)
    Colorectal cancer (CRC) patients with APC mutations do not benefit from 5-FU therapy. It was reported that APC physically interacts with POLβ and FEN1, thus blocking LP-BER via APC's DNA repair inhibitory (DRI) domain in vitro. The aim of this
    Nathaniel S Rial et al.
    International journal of cancer, 124(10), 2270-2280 (2009-01-29)
    Elevated deoxycholic acid (DCA), mutations in the adenomatous polyposis coli (APC) gene and chronic inflammation are associated with increased risk of colorectal cancer. APC status was manipulated to determine whether DCA mediates inflammatory molecules in normal or initiated colonic mucosa.
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