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MAB5310

Sigma-Aldrich

Anti-Glutathione Antibody, clone D8

clone D8, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

D8, monoclonal

species reactivity

eukaryotes

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

isotype

IgG2a

shipped in

dry ice

target post-translational modification

unmodified

Specificity

Reacts with reduced or non-reduced glutathione on proteins only. Does not react with free glutathione.

Immunogen

Glutathione-protein complexes.

Application

Anti-Glutathione Antibody, clone D8 is an antibody against Glutathione for use in WB.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
Western blotting of glutatione conjugated proteins under non-reduced conditions: 1:500-1:1000; does not react with glutatione by itself.

Immunocytochemistry: 1:500-1:1000 (Non-reducing conditions)

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Immunoglobulin purified by protein A affinity and presented as a liquid in PBS, pH 7.2 with 0.01% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Omid Azimzadeh et al.
Cancers, 15(13) (2023-07-14)
Recent epidemiologic studies support an association between chronic low-dose radiation exposure and the development of cardiovascular disease (CVD). The molecular mechanisms underlying the adverse effect of chronic low dose exposure are not fully understood. To address this issue, we have
Vladimir A Mitkevich et al.
Oxidative medicine and cellular longevity, 2016, 9092328-9092328 (2016-05-31)
Many viruses induce oxidative stress and cause S-glutathionylation of Cys residues of the host and viral proteins. Changes in cell functioning during viral infection may be associated with glutathionylation of a number of key proteins including Na,K-ATPase which creates a
Yugo Tsuchiya et al.
The Biochemical journal, 474(14), 2489-2508 (2017-03-28)
Coenzyme A (CoA) is an obligatory cofactor in all branches of life. CoA and its derivatives are involved in major metabolic pathways, allosteric interactions and the regulation of gene expression. Abnormal biosynthesis and homeostasis of CoA and its derivatives have
S-glutathionylation of the Na,K-ATPase catalytic ? subunit is a determinant of the enzyme redox sensitivity.
Petrushanko, IY; Yakushev, S; Mitkevich, VA; Kamanina, YV; Ziganshin, RH; Meng et al.
The Journal of Biological Chemistry null
Ursolic acid protects monocytes against metabolic stress-induced priming and dysfunction by preventing the induction of Nox4.
Ullevig, SL; Kim, HS; Nguyen, HN; Hambright, WS; Robles, AJ; Tavakoli, S; Asmis, R
Redox Biology null

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