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ABE1026

Sigma-Aldrich

Anti-SMARCA5 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

SMARCA5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5, Sucrose nonfermenting protein 2 homolog, hSNF2H

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_003592

UniProt accession no.

O60264

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SMARCA5(8467)

General description

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 is a protein encoded by the SMARCA5 gene in human. A member of the SWI/SNF family proteins with helicase and ATPase activities that are thought to regulate transcriptions by altering the chromatin structure around the target genes.

Immunogen

Epitope: Near N-terminus
GST-tagged recombinant protein corresponding to human SMARCA5 near the N-terminus.

Application

Anti-SMARCA5 Antibody is an antibody against SMARCA5 for use in Western Blotting and Immunocytochemistry, Immunoprecipitation.
Immunocytochemistry Analysis: 2.0 ug/mL from a representative lot detected SMARCA5 in HeLa, A431, and NIH/3T3 cells.
Immunoprecipitation Analysis: 5.0 ug from a representative lot immunoprecipitated SMARCA5 in 500 µg of HeLa cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected SMARCA5 in 10 µg of HeLa cell lysate.

Target description

~130 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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