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Key Documents

AB1506

Sigma-Aldrich

Anti-Glutamate Receptor 2 & 3 Antibody

Chemicon®, from rabbit

Synonym(s):

AMPA-selective glutamate receptor 2, AMPA-selective glutamate receptor 2, glutamate receptor 2, glutamate receptor, ionotropic, AMPA 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

hamster, mouse, human, guinea pig, monkey, gerbil, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRIA2(2891)

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcategorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition.

Specificity

Expected to cross-react with human, rat, monkey, gerbil, guinea pig, and hamster.
It recognizes both GluR2 and GluR3 which have nearly identical carboxy terminal sequences. Western blot analysis of rat brain shows two poorly separated bands which comigrate with GluR2 and GluR3 expressed in transfected cells. GluR2 migrates slightly ahead of GluR3. No cross-reaction with GluR1 or GluR4. The antibody appears to recognize GluR 2 & 3 in several other species, but has not been extensively characterized in these cases.

Immunogen

Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI).
Epitope: C-terminus

Application

Immunohistochemistry:
A previous lot was used on paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 µg/mL.

Immunoprecipitation:
When linked to immobilized Protein A, a previous lot of this antibody immunoprecipitated detergent-solubilized receptor from brain or transfected cells.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Glutamate Receptor 2 & 3 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 2 & 3.

Quality

Evaluated by Western Blot on rat brain lysate.

Western Blot Analysis:
1:1000 dilution of this antibody detected glutamate receptor 2 & 3 on 10 μg of rat brain lysate.

Target description

108 kDa

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal serum lyophilized from buffer containing 0.01M phosphate, 0.09M NaCl, pH 7.2. 1% BSA and 0.1% sodium azide.

Storage and Stability

Maintain lyophilized material at +2-8°C or -20°C for up to 6 months after date of receipt.
Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks at +2-8°C and reused repeatedly.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Human brain tissue and extracts from mouse brain and rat brain tissue.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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ZO-1 and the spatial organization of gap junctions and glutamate receptors in the outer plexiform layer of the mammalian retina.
Puller, C; de Sevilla Muller, LP; Janssen-Bienhold, U; Haverkamp, S
The Journal of Neuroscience null
Eunice Y Yuen et al.
The Journal of physiology, 588(Pt 13), 2361-2371 (2010-05-06)
Cofilin, the major actin depolymerizing factor, modulates actin dynamics that contribute to spine morphology, synaptic transmission and plasticity. Much evidence implicates the cofilin inactivation kinase LIMK in synaptic function, but little is known about the cofilin activation phosphatase Slingshot in
Suleman Hussain et al.
Frontiers in molecular neuroscience, 9, 10-10 (2016-02-24)
Syntaxins are a family of membrane-integrated proteins that are instrumental in exocytosis of vesicles. Syntaxin-1 is an essential component of the presynaptic exocytotic fusion machinery in the brain and interacts with several other proteins. Syntaxin-1 forms a four-helical bundle complex
Amanda L Jacob et al.
Hippocampus, 25(7), 798-812 (2014-12-20)
AMPA receptors are the principal mediators of excitatory synaptic transmission in the mammalian central nervous system. The subunit composition of these tetrameric receptors helps to define their functional properties, and may also influence the synaptic trafficking implicated in long-term synaptic
Morgane Boillot et al.
Scientific reports, 6, 21769-21769 (2016-02-18)
The secreted leucine-rich glioma inactivated 1 (LGI1) protein is an important actor for human seizures of both genetic and autoimmune etiology: mutations in LGI1 cause inherited temporal lobe epilepsy, while LGI1 is involved in antibody-mediated encephalitis. Remarkably, Lgi1-deficient (Lgi1(-/-)) mice

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