1.13301
Bactident™ Aminopeptidase
suitable for detection, pkg of 50 strips
Synonym(s):
Test on L-alanine aminopeptidase
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About This Item
Recommended Products
feature
ready-to-use
Quality Level
packaging
pkg of 50 strips
manufacturer/tradename
Bactident™
technique(s)
microbe id | specific enzyme detection: suitable
antibiotic activity spectrum
Gram-negative bacteria
application(s)
detection
environmental
food and beverages
storage temp.
2-8°C
suitability
Aeromonas spp.
Bacillus spp.
Salmonella spp.
General description
BACTIDENT™ Aminopeptidase test strips are diagnostic test strips used in microbiology to detect the activity of aminopeptidase enzymes in bacterial strains. Aminopeptidases are a group of enzymes that cleave amino acids from the amino terminus of polypeptides and proteins. The test strips contain L-alanine-4-nitroanilide as a substrate, which is split into 4-nitroaniline and the amino acid L-alanine in the presence of the enzyme alanine aminotransferase. The L-alanine aminopeptidase enzyme is localized in the cell envelope of bacteria, and the development of clear yellow coloration of 4-nitroaniline indicates the presence of the enzyme.
Application
BACTIDENT™ Aminopeptidase test strips are useful for bioburden testing in the industry. The aminopeptidase test is a reliable method for determining the Gram behavior and differentiating Gram-positive from Gram-negative bacteria. These test strips are used for the detection of L-alanine aminopeptidase-producing microorganisms. The test is particularly useful in identifying Enterobacteriaceae and in differentiating between species of clinically important bacteria such as Streptococcus and Enterococcus.
Linkage
Replaces: 1.13301.0002
Analysis Note
Colour change to yellow
- Salmonella typhimurium ATCC 14028 (WDCM 00031):
+
- Aeromonas hydrophila ATCC 7966 (WDCM 00063): +
- Proteus rettgeri ATCC 29944: +
- Yersinia enterocolitica ATCC 9610 (WDCM 00038): +
- Bacillus cereus ATCC 11778 (WDCM 00001): -
- Staphylococcus aureus ATCC 25923 (WDCM 00034): -
- Enterococcus faecalis ATCC 11700: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031):
+
- Aeromonas hydrophila ATCC 7966 (WDCM 00063): +
- Proteus rettgeri ATCC 29944: +
- Yersinia enterocolitica ATCC 9610 (WDCM 00038): +
- Bacillus cereus ATCC 11778 (WDCM 00001): -
- Staphylococcus aureus ATCC 25923 (WDCM 00034): -
- Enterococcus faecalis ATCC 11700: -
Legal Information
BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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