1.11723
NutriSelect® plus Casein-peptone lecithin polysorbate broth (base)
USP, For sterility tests of viscous or gelatinous materials
Synonym(s):
TAT broth
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About This Item
Agency
USP
Quality Level
sterility
non-sterile
form
powder
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
application(s)
cosmetics
microbiology
pharmaceutical
storage temp.
15-25°C
General description
Casein-peptone lecithin polysorbate broth (base) is a granulated medium recommended for general microbiological analysis. The granules ensure rapid and uniform dissolution in water, prevent clumping of the medium, and inhalation of the airborne powder. Casein enzymic hydrolysate provides the nitrogen, vitamins, amino acids, and carbon in the broth base. Casein peptone in the medium present optimal conditions for the spore germination and regeneration of the damaged microorganisms. Lecithin and polysorbate 20 inactivate many antimicrobial compounds.
Application
Casein-peptone lecithin polysorbate broth (base) is recommended for diluting samples of pharmaceutical, cosmetic, and other raw materials or final products for determining the total microbial counts.
Analysis Note
Appearance (clearness): clear to almost clear
Appearance (colour): yellowish
pH-value (25 °C): 6.9 - 7.3
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): +
Growth (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): +
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): +
Growth (Candida albicans ATCC 10231 (WDCM 00054)): +
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): +
Incubation: 24 hrs.; 35 °C; aerobic;
Aspergillus und C.albicans up to 3 days; 20 - 25 °C; aerobic .
Appearance (colour): yellowish
pH-value (25 °C): 6.9 - 7.3
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): +
Growth (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): +
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): +
Growth (Candida albicans ATCC 10231 (WDCM 00054)): +
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): +
Incubation: 24 hrs.; 35 °C; aerobic;
Aspergillus und C.albicans up to 3 days; 20 - 25 °C; aerobic .
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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