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V8137

Sigma-Aldrich

Anti-V5 antibody produced in rabbit

IgG fraction of antiserum

Synonym(s):

Rabbit Anti-V5

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About This Item

MDL number:
MFCD03456076
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

technique(s)

immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells
immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells
western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Anti-V5 antibody produced in rabbit has been used in:
  • immunocytochemistry
  • immunoprecipitation
  • western blot

Physical form

Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Storage and Stability

Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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