P3902

Anti-Pyk2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CAK-β, Anti-Proline rich Kinase 2
• MDL number: MFCD01634069
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 110-116 kDa
• species reactivity: rat, mouse, human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections of human cerebellum.; immunoprecipitation (IP): 3-5 μg/mL using 150-200 μg of PC-12 rat pheochromocytoma RIPA lysate; microarray: suitable; western blot: 1:2,000 using a whole extract of LPS-stimulated P388 mouse monocyte-macrophage cells
• UniProt accession no.: Q14289
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PTK2B(2185) ; mouse ... Ptk2b(19229) ; rat ... Ptk2b(50646)

Description

General description

Pyk2 (proline-rich kinase 2) protein belongs to tyrosine protein kinase family and is primarily expressed in the central nervous system and in cells derived from hematopoietic lineages. Pyk2 is found in tissues and cells like mesenchymal, epithelial, endothelial cells, neonatal cardiomyocytes, osteoclasts and neuronal cells.

Immunogen

Synthetic peptide corresponding to amino acid residues 991-1009 of human Pyk2, coupled to KLH. This sequence is highly conserved in rat and mouse (1 amino acid substitution).

Application

Anti-Pyk2 antibody produced in rabbit has been used in:

• immunocytochemistry
• immunohistochemistry
• western blotting

Biochem/physiol Actions

Protein tyrosine kinases (PTKs) are critical components of the signalling pathways that control cell growth, differentiation, apoptosis, metabolism, cell cycle regulation and cytoskeletal function. Pyk2 has been detected in cell-cell contacts, at focal adhesion-like structures and podosomes, cytoplasmic perinuclear region, in association with actin filaments and diffusely distributed in the cytoplasm. Pyk2 phosphorylation is critical for its interaction with SH2-containing signalling molecules and their linkage to signalling pathways that regulate extracellular-signal-regulated kinase (ERK), Janus kinases (JNK) and p38 kinases. Pyk2 has been shown to interact with Src family kinases, the growth factor receptor-bound protein 2 (Grb2)/Sos complex, p130cas, paxillin, Hic-5, and several other proteins, including inhibitors, to regulate signalling as well as cytoskeletal and morphological changes of cells. It has a crucial role in T and B cell antigen receptor signaling, cell cycle progression, metastasis, NK cytotoxicity, modulation of ion channel function and neuronal short- and long- term responses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable