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MSAB001

Sigma-Aldrich

SILu™Rich Anti-IL6 MAb, biotin conjugated

clone IL6-4G1, purified from hybridoma cell culture

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

Quality Level

200

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

IL6-4G1, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: suitable
immunoprecipitation (IP): 1-2 μg/test using concentrated supernatant of human osteosarcoma MG-63 cells induced with IL 1β

isotype

IgG1

UniProt accession no.

P05231

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... IL6(3569)

Immunogen

Recombinant IL6

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4 containing 15 mM sodium azide

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regine M Schoenherr et al.
Proteomics, 16(15-16), 2141-2145 (2016-04-21)
Immunoaffinity enrichment of peptides coupled to multiple reaction monitoring-mass spectrometry (immuno-MRM) enables highly specific, sensitive, and precise quantification of peptides and post-translational modifications. Major obstacles to developing a large number of immuno-MRM assays are poor availability of monoclonal antibodies (mAbs)
Ailan Guo et al.
Molecular & cellular proteomics : MCP, 13(1), 372-387 (2013-10-17)
Protein methylation is a common posttranslational modification that mostly occurs on arginine and lysine residues. Arginine methylation has been reported to regulate RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Lysine methylation is best known to
Tove Boström et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1021, 3-13 (2015-11-14)
For protein analysis of biological samples, two major strategies are used today; mass spectrometry (MS) and antibody-based methods. Each strategy offers advantages and drawbacks. However, combining the two using an immunoenrichment step with MS analysis brings together the benefits of
Luca Semerano et al.
Expert opinion on investigational drugs, 23(7), 979-999 (2014-04-29)
IL-6 is a key cytokine in the pathogenesis of rheumatoid arthritis (RA). The clinical efficacy of tocilizumab (TCZ), a humanized anti-IL6-receptor mAb, confirmed the value of IL-6 blockade in this disease. A number of new anti-IL-6 biologics are currently in
P M Ridker et al.
The New England journal of medicine, 336(14), 973-979 (1997-04-03)
Inflammation may be important in the pathogenesis of atherothrombosis. We studied whether inflammation increases the risk of a first thrombotic event and whether treatment with aspirin decreases the risk. We measured plasma C-reactive protein, a marker for systemic inflammation, in
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