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G0774

Sigma-Aldrich

Glycerokinase from Bacillus stearothermophilus

buffered aqueous solution, ≥75 units/mg protein (biuret)

Synonym(s):

ATP:glycerol 3-phosphotransferase, Glycerol Kinase

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About This Item

CAS Number:
9030-66-4
Enzyme Commission number:
2.7.1.30 (BRENDA, IUBMB)
EC Number:
232-862-0
MDL number:
MFCD00131205
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Bacillus sp. (Bacillus steaarothermophilus)

Assay

≥5.0 mg protein/mL (biuret)

form

buffered aqueous solution

specific activity

≥75 units/mg protein (biuret)

storage condition

dry at room temperature

color

beige

application(s)

life science and biopharma

storage temp.

2-8°C

Gene Information

Bacillus sp. ... glpK(89613566)

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General description

Research area: Cell Signaling

Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.

Application

Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.

Unit Definition

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Physical form

Stabilized solution in Tris buffer, pH 7.3

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Carol J Hartley et al.
PloS one, 12(11), e0184183-e0184183 (2017-11-08)
Carbon-carbon bond formation is one of the most challenging reactions in synthetic organic chemistry, and aldol reactions catalysed by dihydroxyacetone phosphate-dependent aldolases provide a powerful biocatalytic tool for combining C-C bond formation with the generation of two new stereo-centres, with
Purification and properties of glycerol kinase from Escherichia coli.
S I Hayashi et al.
The Journal of biological chemistry, 242(5), 1030-1035 (1967-03-10)
N Zwaig et al.
Science (New York, N.Y.), 153(3737), 755-757 (1966-08-12)
Fructose-1 ,6-diphosphate is a feedback inhibitor of the catabolic enzyme, glycerol kinase, in Escherichia coli. A mutant was isolated which produced a desensitized enzyme. Glucose was no longer as effective in preventing the utilization of exogenous glycerol by cells which
Mitsuko Ohashi-Suzuki et al.
The Journal of veterinary medical science, 73(5), 615-621 (2010-12-29)
African trypanosome species are causative agents for sleeping sickness in humans and nagana disease in cattle. Trypanosoma brucei can generate ATP via a reverse reaction with glycerol kinase (GK) when alternative oxidase (AOX) is inhibited; thus, GK is considered to
Anesthesia for a child suffering from a deletion in the Xp21 loci resulting in Duchenne disease, glycerol kinase deficiency, and congenital adrenal hypoplasia.
Luc J Van Obbergh et al.
Paediatric anaesthesia, 21(10), 1085-1087 (2011-10-11)
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